Nitrogen and sulfur for phosphorus: Lipidome adaptation of anaerobic sulfate- reducing bacteria in phosphorus- deprived conditions

• 成果类型: Article
• 署名作者: Ding, Su; Grossi, Vincent; Hopmans, Ellen C.; Bale, Nicole J.; Laureau, Cristiana Cravo-; Damste, Jaap S. Sinninghe
• 署名单位: Utrecht University; Royal Netherlands Institute for Sea Research (NIOZ); Ecole Normale Superieure de Lyon (ENS de LYON); Centre National de la Recherche Scientifique (CNRS); Universite Claude Bernard Lyon 1; Universite de Pau et des Pays de l'Adour; Centre National de la Recherche Scientifique (CNRS); Utrecht University; Centre National de la Recherche Scientifique (CNRS); Aix-Marseille Universite
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-9781
• DOI: 10.1073/pnas.2400711121
• 发表日期: 2024-06-11
• 关键词: intact polar lipids; membrane-lipids; mass-spectrometry; diabolic acid; water column; surface; biosynthesis; temperature; biomarkers; diversity

摘要

largely unexplored. In this study, we thoroughly examined the lipidome adaptations of bacterium known for its high proportions of alkylglycerol ether lipids in its membrane, under various cultivation conditions including temperature, pH, salinity, and ammonium and phosphorous concentrations. Employing an extensive analytical and computational lipidomic methodology, we identified an assemblage of nearly 400 distinct lipids, including a range of glycerol ether/ester lipids with various polar head groups. Information theory- based analysis revealed that temperature fluctuations and phosphate scarcity profoundly influenced the lipidome's composition, leading to an enhanced diversity and specificity of novel lipids. Notably, phosphorous limitation led to the biosynthesis of novel glucuronosylglycerols and sulfur- containing aminolipids, termed butyramide cysteine glycerols, featuring various ether/ester bonds. This suggests a novel adaptive strategy for anaerobic heterotrophs to thrive under phosphorus- depleted conditions, characterized by a diverse array of nitrogen- and sulfur- containing polar head groups, moving beyond a reliance on conventional nonphospholipid types.