A receptor for dual ligands governs plant immunity and hormone response and is targeted by a nematode effector

• 成果类型: Article
• 署名作者: Huang, Li; Yuan, Yulin; Ramirez, Chloe; Zhao, Zhe; Chen, Letian; Griebel, Thomas; Kud, Joanna; Kuhl, Joseph C.; Caplan, Allan; Dandurand, Louise-Marie; Xiao, Fangming
• 署名单位: University of Idaho; South China Agricultural University; Guangdong Laboratory for Lingnan Modern Agriculture; Free University of Berlin; University of Arkansas System; University of Arkansas Fayetteville; University of Idaho
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-9726
• DOI: 10.1073/pnas.2412016121
• 发表日期: 2024-10-10
• 关键词: root-knot nematodes; perception

摘要

In this study, we show that the potato ( Solanum tuberosum) pattern recognition receptor (PRR) NEMATODE- INDUCED LEUCINE-RICH REPEAT (LRR)-RLK1 (StNILR1) functions as a dual receptor, recognizing both nematode- associated molecular pattern ascaroside #18 (Ascr18) and plant hormone brassinosteroid (BR) to activate two different physiological outputs: pattern- triggered immunity (PTI) and BR response. Ascr18/BR- StNILR1 signaling requires the coreceptor potato BRASSINOSTEROID INSENSITIVE 1- ASSOCIATED RECEPTOR KINASE 1 (StBAK1) and perception of either ligand strengthens StNILR1 interaction with StBAK1 in plant cells. Significantly, the parasitically successful potato cyst nematode ( Globodera pallida) utilizes the effector RHA1B, which is a functional ubiquitin ligase, to target StNILR1 for ubiquitination- mediated proteasome- dependent degradation, thereby countering Ascr18/BR- StNILR1- mediated PTI in potato and facilitating nematode parasitism. These findings broaden our understanding of PRR specificity and reveal a nematode parasitic mechanism that targets a PTI signaling pathway. Significance This study reveals a previously undescribed mechanism in plant defense, wherein a single pattern recognition receptor(StNILR1) recognizes a nematode- associated molecular pattern (NAMP) ascaroside #18 (Ascr18) as well as the phytohormone brassinosteroid (BR) to trigger distinct physiological responses. Furthermore, we identify a nematode effector (RHA1B) that disrupts this dual signaling by targeting StNILR1 for degradation. These findings shed light on the intricate interplay between plant immunity, hormonal regulation, and nematode parasitism.