Cascaded momentum-space polarization filters enabled label-free black-field microscopy for single nanoparticles analysis

• 成果类型: Article
• 署名作者: Liu, Yang; Chen, Qiankun; Zhang, Hongli; Feng, Zeyu; Zou, Gang; Zhang, Douguo
• 署名单位: Chinese Academy of Sciences; University of Science & Technology of China, CAS; Chinese Academy of Sciences; University of Science & Technology of China, CAS; Chinese Academy of Sciences; University of Science & Technology of China, CAS; Hefei National Laboratory; Chinese Academy of Sciences; University of Science & Technology of China, CAS
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-8873
• DOI: 10.1073/pnas.2321825121
• 发表日期: 2024-03-26
• 关键词: interferometric scattering microscopy; halide perovskites cspbx3; ion dynamics; interference; tracking; nanocrystals; proteins; br; cl

摘要

Label-free optical imaging of single- nanometer-scale matter is extremely important for a variety of biomedical, physical, and chemical investigations. One central challenge is that the background intensity is much stronger than the intensity of the scattering light from single nano- objects. Here, we propose an optical module comprising cascaded momentum-space polarization filters that can perform vector field modulation to block most of the background field and result in an almost black background; in contrast, only a small proportion of the scattering field is blocked, leading to obvious imaging contrast enhancement. This module can be installed in various optical microscopies to realize a black-field microscopy. Various single nano- objects with dimensions smaller than 20 nm appear distinctly in the black-field images. The chemical reactions occurring on single nanocrystals with edge lengths of approximately 10 nm are in situ real-time monitored by using the black-field microscopy. This label-free black-field microscopy is highly promising for a wide range of future multidisciplinary science applications.