Structural and mechanistic basis of the central energy- converting methyltransferase complex of methanogenesis

• 成果类型: Article
• 署名作者: Aziz, Iram; Kayastha, Kanwal; Kaltwasser, Susann; Vonck, Janet; Welsch, Sonja; Murphy, Bonnie J.; Kahnt, Jorg; Wu, Di; Wagner, Tristan; Shima, Seigo; Ermler, Ulrich
• 署名单位: University of Punjab; Leiden University; Max Planck Society; Max Planck Society; Max Planck Society; Max Planck Society; Max Planck Society; Max Planck Society
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-8645
• DOI: 10.1073/pnas.2315568121
• 发表日期: 2024-04-02
• 关键词: coenzyme-m methyltransferase; n-5-methyltetrahydromethanopterincoenzyme m methyltransferase; methanobacterium-thermoautotrophicum; protein; energetics; n-5-methyl-tetrahydromethanopterin; biochemistry; TOPOLOGY; insights; archaea

摘要

Methanogenic archaea inhabiting anaerobic environments play a crucial role in the global biogeochemical material cycle. The most universal electrogenic reaction of their methane- producing energy metabolism is catalyzed byN5- methyl- tetrahydromethanopterin: coenzyme M methyltransferase (MtrABCDEFGH), which couples the vectorial Na+ transport with a methyl transfer between the one- carbon carriers tetrahydromethanopterin and coenzyme M via a vitamin B12 derivative (cobamide) as prosthetic group. We present the 2.08 & Aring; cryo-EM structure of Mtr(ABCDEFG)3 composed of the central Mtr(ABFG)3 stalk symmetrically flanked by three membrane- spanning MtrCDE globes. Tetraether glycolipids visible in the map fill gaps inside the multisubunit complex. Putative coenzyme M and Na+ were identified inside or in a side- pocket of a cytoplasmic cavity formed within MtrCDE. Its bottom marks the gate of the transmembrane pore occluded in the cryo-EM map. By integrating Alphafold2 information, functionally competent MtrA-MtrH and MtrA-MtrCDE subcomplexes could be modeled and thus the methyl- tetrahydromethanopterin demethylation and coenzyme M methylation half- reactions structurally described. Methyl- transfer- driven Na+ transport is proposed to be based on a strong and weak complex between MtrCDE and MtrA carrying vitamin B12, the latter being placed at the entrance of the cytoplasmic MtrCDE cavity. Hypothetically, strongly attached methyl- cob(III)amide (His - on) carrying MtrA induces an inward- facing conformation, Na+ flux into the membrane protein center and finally coenzyme M methylation while the generated loosely attached (or detached) MtrA carrying cob(I)amide (His - off) induces an outward- facing conformation and an extracellular Na+ outflux. Methyl- cob(III)amide (His - on) is regenerated in the distant active site of the methyl- tetrahydromethanopterin binding MtrH implicating a large - scale shuttling movement of the vitamin B12 - carrying domain.