Self- assembling dendrimer nanosystems for specific fluorine magnetic resonance imaging and effective theranostic treatment of tumors

• 成果类型: Article
• 署名作者: Lyu, Zhenbin; Ralahy, Brigino; Barbacaru, Teodora- Adriana Perles-; Ding, Ling; Jiang, Yifan; Lian, Baoping; Roussel, Tom; Liu, Xi; Galanakou, Christina; Laurini, Erik; Tintaru, Aura; Giorgio, Suzanne; Pricl, Sabrina; Liu, Xiaoxuan; Bernard, Monique; Iovanna, Juan; Viola, Angele; Peng, Ling
• 署名单位: Aix-Marseille Universite; Centre National de la Recherche Scientifique (CNRS); Aix-Marseille Universite; Centre National de la Recherche Scientifique (CNRS); CNRS - Institute of Chemistry (INC); Aix-Marseille Universite; Centre National de la Recherche Scientifique (CNRS); CNRS - Institute for Engineering & Systems Sciences (INSIS); China Pharmaceutical University; China Pharmaceutical University; Institut National de la Sante et de la Recherche Medicale (Inserm); Aix-Marseille Universite; UNICANCER; Institut Paoli-Calmette (IPC); Centre National de la Recherche Scientifique (CNRS); CNRS - National Institute for Biology (INSB); University of Trieste; University of Lodz
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-8613
• DOI: 10.1073/pnas.2322403121
• 发表日期: 2024-06-18
• 关键词: amphiphilic dendrimer; contrast-agents; f-19; DESIGN; mri; nanoparticles; delivery; rna

摘要

Fluorine magnetic resonance imaging ( 19 F - MRI) is particularly promising for biomedical applications owing to the absence of fluorine in most biological systems. However, its use has been limited by the lack of safe and water - soluble imaging agents with high fluorine contents and suitable relaxation properties. We report innovative 19 F - MRI agents based on supramolecular dendrimers self - assembled by an amphiphilic dendrimer composed of a hydrophobic alkyl chain and a hydrophilic dendron. Specifically, this amphiphilic dendrimer bears multiple negatively charged terminals with high fluorine content, which effectively prevented intra - and intermolecular aggregation of fluorinated entities via electrostatic repulsion. This permitted high fluorine nuclei mobility alongside good water solubility with favorable relaxation properties for use in 19 F - MRI. Importantly, the self - assembling 19 F - MRI agent was able to encapsulate the near - infrared fluorescence (NIRF) agent DiR and the anticancer drug paclitaxel for multimodal 19 F - MRI and NIRF imaging of and theranostics for pancreatic cancer, a deadly disease for which there remains no adequate early detection method or efficacious treatment. The 19 F - MRI and multimodal 19 F - MRI and NIRF imaging studies on human pancreatic cancer xenografts in mice confirmed the capability of both imaging modalities to specifically image the tumors and demonstrated the efficacy of the theranostic agent in cancer treatment, largely outperforming the clinical anticancer drug paclitaxel. Consequently, these dendrimer nanosystems constitute promising 19 F - MRI agents for effective cancer management. This study offers a broad avenue to the construction of 19 F - MRI agents and theranostics, exploiting self - assembling supramolecular dendrimer chemistry.