Insulin receptor orchestrates kidney antibacterial defenses

• 成果类型: Article
• 署名作者: Schwartz, Laura; Simoni, Aaron; Yan, Pearlly; Salamon, Kristin; Turkoglu, Altan; Martinez, Gabriela Vasquez; Zepeda-Orozco, Diana; Eichler, Tad; Wang, Xin; Spencer, John David
• 署名单位: University System of Ohio; Ohio State University; Nationwide Childrens Hospital; University System of Ohio; Ohio State University; Nationwide Childrens Hospital; University System of Ohio; Ohio State University; James Cancer Hospital & Solove Research Institute; University System of Ohio; Ohio State University; James Cancer Hospital & Solove Research Institute; University System of Ohio; Ohio State University
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-8604
• DOI: 10.1073/pnas.2400666121
• 发表日期: 2024-07-16
• 关键词: urinary-tract-infection; duct epithelial-cells; escherichia-coli; intercalated cells; blood-pressure; ribonuclease 7; host-defense; kappa-b; expression; translation

摘要

Urinary tract infection (UTI) commonly afflicts people with diabetes. This augmented infection risk is partly due to deregulated insulin receptor (IR) signaling in the kidney collecting duct. The collecting duct is composed of intercalated cells (ICs) and principal cells (PCs). Evidence suggests that ICs contribute to UTI defenses. Here, we interrogate how IR deletion in ICs impacts antibacterial defenses against uropathogenic Escherichia coli. We also explore how IR deletion affects immune responses in neighboring PCs with intact IR expression. To accomplish this objective, we profile the transcriptomes of IC and PC populations enriched from kidneys of wild- type and IC-- specific IR knock- out mice that have increased UTI susceptibility. Transcriptomic analysis demonstrates that IR deletion suppresses IC-- integrated stress responses and innate immune defenses. To define how IR shapes these immune defenses, we employ murine and human kidney cultures. When challenged with bacteria, murine ICs and human kidney cells with deregulated IR signaling cannot engage central components of the integrated stress response-including activating transcriptional factor 4 (ATF4). Silencing ATF4 impairs NFkB activation and promotes infection. In turn, NFkB silencing augments infection and suppresses antimicrobial peptide expression. In diabetic mice and people with diabetes, collecting duct cells show reduced IR expression, impaired integrated stress response engagement, and compromised immunity. Collectively, these translational data illustrate how IR orchestrates collecting duct antibacterial responses and the communication between ICs and PCs.