Active fluctuations of axoneme oscillations scale with number of dynein motors

• 成果类型: Article
• 署名作者: Sharma, Abhimanyu; Friedrich, Benjamin M.; Geyer, Veikko F.
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-8562
• DOI: 10.1073/pnas.2406244121
• 发表日期: 2024-11-12
• 关键词: chlamydomonas-reinhardtii; flagella; inner; arm; consumption; precision; mechanism; MOVEMENT; tracking; mutants

摘要

Fluxes of energy generate active forces in living matter, yet also active fluctuations. As a canonical example, collections of molecular motors exhibit spontaneous oscillations with frequency jitter caused by nonequilibrium phase fluctuations. We investigate phase fluctuations in reactivated Chlamydomonas reinhardtii axonemes, which are accessible to direct manipulation. We quantify the precision of axonemal oscillations after controlled chemical removal of dynein motors, providing an experimental test for the theory prediction that the quality factor of motor oscillations should increase with motor number. Our quantification reveals specialized roles of inner and outer arm dynein motors. This supports a model in which inner dyneins serve as master pace-makers, to which outer arm dyneins become entrained, consistent with recent insight provided by structural biology.