Conserved C143 forms a branched intermediate in Hedgehog autoprocessing: A cancer drug discovery target against Hedgehog signaling

• 成果类型: Article
• 署名作者: Faris, Shannon; Xia, Ke; Wagner, Andrew G.; Xu, Zihan; Smith, Nathan; Giner, Jose-Luis; Callahan, Brian; Xie, Jian; Wang, Chunyu
• 署名单位: Rensselaer Polytechnic Institute; Rensselaer Polytechnic Institute; State University of New York (SUNY) System; Binghamton University, SUNY; State University of New York (SUNY) System; State University of New York (SUNY) College of Environmental Science & Forestry; University of Nebraska System; University of Nebraska Medical Center
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-14780
• DOI: 10.1073/pnas.2415144122
• 发表日期: 2025-04-29
• 关键词: cerevisiae vma intein; cell growth; PATHWAY; inhibitors; steps; identification; resistance

摘要

Hedgehog (Hh) signaling plays fundamental roles in embryonic development while its abnormal activation in adults is associated with cancer. Hh targeting drugs have gained FDA approval but resistance emerged quickly, underlining the need for novel types of Hh inhibitors. Hh signaling is initiated by the Hh ligand, generated from the autoprocessing of Hh precursor. However, the catalytic role of a highly conserved Hedgehog residue C143 is still poorly understood. Here, we confirmed that C143 is required for Hh autoprocessing in mammalian cells. NMR titration showed that C143 has an extremely low pKa of 4.5, befitting a highly reactive catalytic residue. We further established that Hh autoprocessing involves a branched intermediate (BI) with two N-termini, formed as a thioester on the C143 sidechain. BI migrates slower than the linear Hh precursor on SDS-PAGE and disappears with DTT treatment. With trypsin digestion and LC-MS/MS, we detected the N-terminal fragment from BI, which is absent from the linear Hh precursor. Therefore, C143 mediates the formation of a BI thioester in Hh autoprocessing, with a catalytic role equivalent to C + 1 in intein splicing. These findings bring us closer to a full mechanistic understanding of Hh autoprocessing while unifying the first two catalytic steps of Hh autoprocessing with intein splicing, its likely evolutionary predecessor. C143 can also serve as a target for covalent drugs for inhibiting Hh signaling in cancer.