Cryptic isoprene emission of soybeans

• 成果类型: Article
• 署名作者: Mostofa, Mohammad Golam; Sahu, Abira; Xu, Yuan; Basrai, Insiya; Doron, Lior; Lefrancois, Violet; Sharkey, Thomas D.
• 署名单位: United States Department of Energy (DOE); Michigan State University; Michigan State University; Michigan State University; State University of New York (SUNY) System; State University of New York (SUNY) College of Environmental Science & Forestry; SUNY Maritime College; University of Alabama System; University of Alabama Birmingham
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-13564
• DOI: 10.1073/pnas.2502360122
• 发表日期: 2025-06-12
• 关键词: biochemical-characterization; diphosphate synthase; hydrogen-peroxide; high-temperature; plants; thermotolerance; photosynthesis; expression; EVOLUTION; protects

摘要

Isoprene is the most abundant nonmethane biogenic hydrocarbon emitted by some plants, mostly trees. It plays critical roles in atmospheric chemistry by contributing to ozone and aerosol formation. Isoprene also benefits plants, particularly under stress, through its signaling roles. Legume crops like soybean were thought to have evolutionarily lost isoprene synthase (ISPS) and are typically considered nonemitters. Here, we report that damage to soybean leaves by wounding or burning triggered a burst of isoprene emission from the undamaged part of the leaves. In silico analysis identified intact ISPS genes in the soybean genome, with features similar to known ISPSs. Protein made from these gene sequences catalyzed isoprene production in the presence of dimethylallyl diphosphate. Isoprene emission in soybeans was linked to reduced photosynthesis rates and stomatal conductance. Metabolomic analysis showed that leaf damage caused a surge in glyceraldehyde 3- phosphate and pyruvate levels, leading to an increase of most of the methylerythritol 4- phosphate pathway metabolites.