De novo phytosterol synthesis in animals

• 成果类型: Article
• 署名作者: Michellod, Dolma; Bien, Tanja; Birgel, Daniel; Violette, Marlene; Kleiner, Manuel; Fearn, Sarah; Zeidler, Caroline; Gruber-Vodicka, Harald R.; Dubilier, Nicole; Liebeke, Manuel
• 署名单位: Max Planck Society; University of Munster; University of Hamburg; North Carolina State University; Imperial College London; University of Bremen; University of Kiel
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-9870
• DOI: 10.1126/science.add7830
• 发表日期: 2023-05-05
• 页码: 520-526
• 关键词: horizontal gene-transfer; sterol composition; beta-sitosterol; bacteria; carbon; methyltransferases; EVOLUTION; symbiosis; triterpenoids; oligochaete

摘要

Sterols are vital for nearly all eukaryotes. Their distribution differs in plants and animals, with phytosterols commonly found in plants whereas most animals are dominated by cholesterol. We show that sitosterol, a common sterol of plants, is the most abundant sterol in gutless marine annelids. Using multiomics, metabolite imaging, heterologous gene expression, and enzyme assays, we show that these animals synthesize sitosterol de novo using a noncanonical C-24 sterol methyltransferase (C24-SMT). This enzyme is essential for sitosterol synthesis in plants, but not known from most bilaterian animals. Our phylogenetic analyses revealed that C24-SMTs are present in representatives of at least five animal phyla, indicating that the synthesis of sterols common to plants is more widespread in animals than currently known.