Viral circular RNA-encoded protein, ceVP28, divulges an antiviral response in invertebrates

• 成果类型: Article
• 署名作者: Limkul, Sirawich; Phiwthong, Tannatorn; Wanvimonsuk, Supitcha; Seabkongseng, Tuangrak; Aunkam, Phirom; Jaree, Phattarunda; Luangtrakul, Waruntorn; Mahanil, Kanjana; Teamtisong, Kamonluck; Tittabutr, Panlada; Teaumroong, Neung; Sarnow, Peter; Wang, Han-Ching; Somboonwiwat, Kunlaya; Boonchuen, Pakpoom
• 署名单位: Suranaree University of Technology; Chulalongkorn University; Mahidol University; Suranaree University of Technology; Stanford University; National Cheng Kung University; National Cheng Kung University
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-9464
• DOI: 10.1073/pnas.2321707122
• 发表日期: 2025-02-25
• 关键词: spot syndrome virus; expression; infection; ires

摘要

Invertebrates mostly use innate immunity to counteract pathogenic infections. In this study, shrimp was used as a model organism to explore the functions of circular RNAs (circRNAs) derived from white spot syndrome virus (WSSV). We identified four viral transcriptomic data of WSSV- infected shrimp. CircVP28, which contains an internal ribosome entry site, was further characterized to determine its potential as a template for protein translation. We observed the presence of a truncated, circRNA- encoded share the same host cell binding partner Rab7, which is a host receptor for WSSV. Coadministration of recombinant ceVP28 protein and WSSV to penaeid shrimps reduced both viral copy numbers and mortality upon WSSV challenges. These findings uncovered a host defense mechanism by which a protein encoded by a viral circRNA modulates virus-receptor interactions, resulting in blocking of viral entry.