Presynaptic Ube3a E3 ligase promotes synapse elimination through down-regulation of BMP signaling

• 成果类型: Article
• 署名作者: Furusawa, Kotaro; Ishii, Kenichi; Tsuji, Masato; Tokumitsu, Nagomi; Hasegawa, Eri; Emoto, Kazuo
• 署名单位: University of Tokyo; University of Tokyo
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-8819
• DOI: 10.1126/science.ade8978
• 发表日期: 2023-09-15
• 页码: 1197-+
• 关键词: angelman-syndrome; ubiquitin ligase; subcellular organization; sensory neuron; active zone; drosophila; insights; protein; GROWTH; gene

摘要

Inactivation of the ubiquitin ligase Ube3a causes the developmental disorder Angelman syndrome, whereas increased Ube3a dosage is associated with autism spectrumdisorders. Despite the enriched localization of Ube3a in the axon terminals including presynapses, little is known about the presynaptic function of Ube3a and mechanisms underlying its presynaptic localization. We show that developmental synapse elimination requires presynaptic Ube3a activity in Drosophila neurons. We further identified the domain of Ube3a that is required for its interaction with the kinesin motor. Angelman syndrome-associated missense mutations in the interaction domain attenuate presynaptic targeting of Ube3a and prevent synapse elimination. Conversely, increased Ube3a activity in presynapses leads to precocious synapse elimination and impairs synaptic transmission. Our findings reveal the physiological role of Ube3a and suggest potential pathogenic mechanisms associated with Ube3a dysregulation.