Whole-brain spatial organization of hippocampal single-neuron projectomes

• 成果类型: Article
• 署名作者: Qiu, Shou; Hu, Yachuang; Huang, Yiming; Gao, Taosha; Wang, Xiaofei; Wang, Danying; Ren, Biyu; Shi, Xiaoxue; Chen, Yu; Wang, Xinran; Wang, Dan; Han, Luyao; Liang, Yikai; Liu, Dechen; Liu, Qingxu; Deng, Li; Chen, Zhaoqin; Zhan, Lijie; Chen, Tianzhi; Huang, Yuzhe; Wu, Qingge; Xie, Taorong; Qian, Liuqin; Jin, Chenxi; Huang, Jiawen; Deng, Wei; Jiang, Tao; Li, Xiangning; Jia, Xueyan; Yuan, Jing; Li, Anan; Yan, Jun; Xu, Ninglong; Xu, Lin; Luo, Qingming; Poo, Mu-Ming; Sun, Yidi; Li, Chengyu T.; Yao, Haishan; Gong, Hui; Sun, Yan-Gang; Xu, Chun
• 署名单位: Chinese Academy of Sciences; Center for Excellence in Brain Science and Intelligence Technology, CAS; Chinese Academy of Sciences; University of Chinese Academy of Sciences, CAS; Lingang Laboratory; ShanghaiTech University; Hainan University; Chinese Academy of Sciences; Kunming Institute of Zoology, CAS; Chinese Academy of Sciences; Kunming Institute of Zoology, CAS; Shanghai Center for Brain Science & Brain-Inspired Technology
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-10427
• DOI: 10.1126/science.adj9198
• 发表日期: 2024-02-02
• 页码: 497-+
• 关键词: pyramidal cells; gabaergic neurons; ca1; projections; circuit; cortex; memory; connections; amygdala

摘要

Mapping single-neuron projections is essential for understanding brain-wide connectivity and diverse functions of the hippocampus (HIP). Here, we reconstructed 10,100 single-neuron projectomes of mouse HIP and classified 43 projectome subtypes with distinct projection patterns. The number of projection targets and axon-tip distribution depended on the soma location along HIP longitudinal and transverse axes. Many projectome subtypes were enriched in specific HIP subdomains defined by spatial transcriptomic profiles. Furthermore, we delineated comprehensive wiring diagrams for HIP neurons projecting exclusively within the HIP formation (HPF) and for those projecting to both intra- and extra-HPF targets. Bihemispheric projecting neurons generally projected to one pair of homologous targets with ipsilateral preference. These organization principles of single-neuron projectomes provide a structural basis for understanding the function of HIP neurons.