Control of cell proliferation by memories of mitosis

• 成果类型: Article
• 署名作者: Meitinger, Franz; Belal, Hazrat; Davis, Robert L.; Martinez, Mallory B.; Shiau, Andrew K.; Oegema, Karen; Desai, Arshad
• 署名单位: University of California System; University of California San Diego; University of California System; University of California San Diego; Ludwig Institute for Cancer Research; Okinawa Institute of Science & Technology Graduate University; Ludwig Institute for Cancer Research
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-12248
• DOI: 10.1126/science.add9528
• 发表日期: 2024-03-29
• 页码: 1441-1448
• 关键词: molecular-mechanisms; kinase inhibitor; centrosome loss; brct domains; dna-damage; 53bp1; p53; phosphorylation; activation; aneuploidy

摘要

Mitotic duration is tightly constrained, and extended mitosis is characteristic of problematic cells prone to chromosome missegregation and genomic instability. We show here that mitotic extension leads to the formation of p53-binding protein 1 (53BP1)-ubiquitin-specific protease 28 (USP28)-p53 protein complexes that are transmitted to, and stably retained by, daughter cells. Complexes assembled through a Polo-like kinase 1-dependent mechanism during extended mitosis and elicited a p53 response in G(1) that prevented the proliferation of the progeny of cells that experienced an approximately threefold extended mitosis or successive less extended mitoses. The ability to monitor mitotic extension was lost in p53-mutant cancers and some p53-wild-type (p53-WT) cancers, consistent with classification of TP53BP1 and USP28 as tumor suppressors. Cancers retaining the ability to monitor mitotic extension exhibited sensitivity to antimitotic agents.