Oxygen imaging of hypoxic pockets in the mouse cerebral cortex

• 成果类型: Article
• 署名作者: Beinlich, Felix R. M.; Asiminas, Antonios; Untiet, Verena; Bojarowska, Zuzanna; Pla, Virginia; Sigurdsson, Bjoern; Timmel, Vincenzo; Gehrig, Lukas; Graber, Michael H.; Hirase, Hajime; Nedergaard, Maiken
• 署名单位: University of Copenhagen; University of Rochester
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-9402
• DOI: 10.1126/science.adn1011
• 发表日期: 2024-03-29
• 页码: 1471-1478
• 关键词: blood-flow; brain; isoflurane; astrocytes; AGE; ph

摘要

Consciousness is lost within seconds upon cessation of cerebral blood flow. The brain cannot store oxygen, and interruption of oxidative phosphorylation is fatal within minutes. Yet only rudimentary knowledge exists regarding cortical partial oxygen tension (Po-2) dynamics under physiological conditions. Here we introduce Green enhanced Nano-lantern (GeNL), a genetically encoded bioluminescent oxygen indicator for Po-2 imaging. In awake behaving mice, we uncover the existence of spontaneous, spatially defined hypoxic pockets and demonstrate their linkage to the abrogation of local capillary flow. Exercise reduced the burden of hypoxic pockets by 52% compared with rest. The study provides insight into cortical oxygen dynamics in awake behaving animals and concurrently establishes a tool to delineate the importance of oxygen tension in physiological processes and neurological diseases.