A naturally isolated symbiotic bacterium suppresses flavivirus transmission by Aedes mosquitoes

• 成果类型: Article
• 署名作者: Zhang, Liming; Wang, Daxi; Shi, Peibo; Li, Juzhen; Niu, Jichen; Chen, Jielong; Wang, Gang; Wu, Linjuan; Chen, Lu; Yang, Zhenxing; Li, Susheng; Meng, Jinxin; Ruan, Fangchao; He, Yuwen; Zhao, Hailong; Ren, Zirui; Wang, Yibaina; Liu, Yang; Shi, Xiaolu; Wang, Yunfu; Liu, Qiyong; Li, Junhua; Wang, Penghua; Wang, Jinglin; Zhu, Yibin; Cheng, Gong
• 署名单位: Tsinghua University; Shenzhen Bay Laboratory; Shenzhen Center for Disease Control & Prevention (SZCDC); Beijing Genomics Institute (BGI); Chinese Academy of Sciences; University of Chinese Academy of Sciences, CAS; Kunming Medical University; Hubei University of Medicine; Chinese Center for Disease Control & Prevention; National Institute for Communicable Disease Control & Prevention, Chinese Center for Disease Control & Prevention; University of Connecticut
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-9203
• DOI: 10.1126/science.adn9524
• 发表日期: 2024-04-19
• 关键词: dengue; microbiota; infection; virus

摘要

The commensal microbiota of the mosquito gut plays a complex role in determining the vector competence for arboviruses. In this study, we identified a bacterium from the gut of field Aedes albopictus mosquitoes named Rosenbergiella sp. YN46 (Rosenbergiella_YN46) that rendered mosquitoes refractory to infection with dengue and Zika viruses. Inoculation of 1.6 x 10(3) colony forming units (CFUs) of Rosenbergiella_YN46 into A. albopictus mosquitoes effectively prevents viral infection. Mechanistically, this bacterium secretes glucose dehydrogenase (RyGDH), which acidifies the gut lumen of fed mosquitoes, causing irreversible conformational changes in the flavivirus envelope protein that prevent viral entry into cells. In semifield conditions, Rosenbergiella_YN46 exhibits effective transstadial transmission in field mosquitoes, which blocks transmission of dengue virus by newly emerged adult mosquitoes. The prevalence of Rosenbergiella_YN46 is greater in mosquitoes from low-dengue areas (52.9 to similar to 91.7%) than in those from dengue-endemic regions (0 to similar to 6.7%). Rosenbergiella_YN46 may offer an effective and safe lead for flavivirus biocontrol.