Homeocurvature adaptation of phospholipids to pressure in deep-sea invertebrates

• 成果类型: Article
• 署名作者: Winnikoff, Jacob R.; Milshteyn, Daniel; Vargas-Urbano, Sasiri J.; Pedraza-Joya, Miguel A.; Armando, Aaron M.; Quehenberger, Oswald; Sodt, Alexander; Gillilan, Richard E.; Dennis, Edward A.; Lyman, Edward; Haddock, Steven H. D.; Budin, Itay
• 署名单位: University of California System; University of California San Diego; Harvard University; Monterey Bay Aquarium Research Institute; University of California System; University of California Santa Cruz; University of Delaware; University of California System; University of California San Diego; National Institutes of Health (NIH) - USA; NIH Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-13453
• DOI: 10.1126/science.adm7607
• 发表日期: 2024-06-28
• 页码: 1482-1488
• 关键词: molecular-dynamics simulations; barotropic phase-transitions; constant-pressure; membrane-fusion; lipid-composition; temperature; gel; energy; water; phosphatidylethanolamine

摘要

Hydrostatic pressure increases with depth in the ocean, but little is known about the molecular bases of biological pressure tolerance. We describe a mode of pressure adaptation in comb jellies (ctenophores) that also constrains these animals' depth range. Structural analysis of deep-sea ctenophore lipids shows that they form a nonbilayer phase at pressures under which the phase is not typically stable. Lipidomics and all-atom simulations identified phospholipids with strong negative spontaneous curvature, including plasmalogens, as a hallmark of deep-adapted membranes that causes this phase behavior. Synthesis of plasmalogens enhanced pressure tolerance in Escherichia coli, whereas low-curvature lipids had the opposite effect. Imaging of ctenophore tissues indicated that the disintegration of deep-sea animals when decompressed could be driven by a phase transition in their phospholipid membranes.