Cross-species single-cell spatial transcriptomic atlases of the cerebellar cortex

• 成果类型: Article
• 署名作者: Hao, Shijie; Zhu, Xiaojia; Huang, Zhi; Yang, Qianqian; Liu, Hean; Wu, Yan; Zhan, Yafeng; Dong, Yu; Li, Chao; Wang, He; Haasdijk, Elize; Wu, Zihan; Li, Shenglong; Yan, Haotian; Zhu, Lijing; Guo, Shiyong; Wang, Zefang; Ye, Aojun; Lin, Youning; Cui, Luman; Tan, Xing; Liu, Huanlin; Wang, Mingli; Chen, Jing; Zhong, Yanqing; Du, Wensi; Wang, Guangling; Lai, Tingting; Cao, Mengdi; Yang, Tao; Xu, Yuanfang; Li, Ling; Yu, Qian; Zhuang, Zhenkun; Xia, Ying; Lei, Ying; An, Yingjie; Cheng, Mengnan; Zhao, Yun; Han, Lei; Yuan, Yue; Song, Xinxiang; Song, Yumo; Gu, Liqin; Liu, Chang; Lin, Xiumei; Wang, Ruiqi; Wang, Zhifeng; Wang, Yang; Li, Shenyu; Li, Huanhuan; Song, Jingjing; Chen, Mengni; Zhou, Wanqiu; Yuan, Nini; Sun, Suhong; Wang, Shiwen; Chen, Yu; Zheng, Mingyuan; Fang, Jiao; Zhang, Ruiyi; Zhang, Shuzhen; Chai, Qinwen; Liu, Jiabing; Wei, Wu; He, Jie; Zhou, Haibo; Sun, Yangang; Liu, Zhen; Liu, Chuanyu; Yao, Jianhua; Liang, Zhifeng; Xu, Xun; Poo, Muming; Li, Chengyu; De Zeeuw, Chris I.; Shen, Zhiming; Liu, Zhiyong; Liu, Longqi; Liu, Shiping; Sun, Yidi; Liu, Cirong
• 署名单位: Chinese Academy of Sciences; Chinese Academy of Sciences; University of Chinese Academy of Sciences, CAS; Chinese Academy of Sciences; Chinese Academy of Sciences; Kunming Institute of Zoology, CAS; Beijing Genomics Institute (BGI); South China University of Technology; Lingang Laboratory; Erasmus University Rotterdam; Erasmus MC; Royal Netherlands Academy of Arts & Sciences; Netherlands Institute for Neuroscience (NIN-KNAW); Tencent; Beijing Genomics Institute (BGI); Shanxi Medical University; Shanghai Center for Brain Science & Brain-Inspired Technology
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-10562
• DOI: 10.1126/science.ado3927
• 发表日期: 2024-09-27
• 关键词: glutamate-receptor delta-2; motor coordination; expression; primate; EVOLUTION; protein

摘要

The molecular and cellular organization of the primate cerebellum remains poorly characterized. We obtained single-cell spatial transcriptomic atlases of macaque, marmoset, and mouse cerebella and identified primate-specific cell subtypes, including Purkinje cells and molecular-layer interneurons, that show different expression of the glutamate ionotropic receptor Delta type subunit 2 (GRID2) gene. Distinct gene expression profiles were found in anterior, posterior, and vestibular regions in all species, whereas region-selective gene expression was predominantly observed in the granular layer of primates and in the Purkinje layer of mice. Gene expression gradients in the cerebellar cortex matched well with functional connectivity gradients revealed with awake functional magnetic resonance imaging, with more lobule-specific differences between primates and mice than between two primate species. These comprehensive atlases and comparative analyses provide the basis for understanding cerebellar evolution and function.