Neuromodulator and neuropeptide sensors and probes for precise circuit interrogation in vivo

• 成果类型: Review
• 署名作者: Muir, J.; Anguiano, M.; Kim, C. K.
• 署名单位: University of California System; University of California Davis; University of California System; University of California Davis
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-8730
• DOI: 10.1126/science.adn6671
• 发表日期: 2024-09-27
• 关键词: scan cyclic voltammetry; dopamine release; neurons; receptors; calcium; brain; transmission; activation; DYNAMICS; norepinephrine

摘要

To determine how neuronal circuits encode and drive behavior, it is often necessary to measure and manipulate different aspects of neurochemical signaling in awake animals. Optogenetics and calcium sensors have paved the way for these types of studies, allowing for the perturbation and readout of spiking activity within genetically defined cell types. However, these methods lack the ability to further disentangle the roles of individual neuromodulator and neuropeptides on circuits and behavior. We review recent advances in chemical biology tools that enable precise spatiotemporal monitoring and control over individual neuroeffectors and their receptors in vivo. We also highlight discoveries enabled by such tools, revealing how these molecules signal across different timescales to drive learning, orchestrate behavioral changes, and modulate circuit activity.