Plant plasmodesmata bridges form through ER-dependent incomplete cytokinesis

• 成果类型: Article
• 署名作者: Li, Ziqiang P.; Moreau, Hortense; Petit, Jules D.; Moraes, Tatiana S.; Smokvarska, Marija; Perez-Sancho, Jessica; Petrel, Melina; Decoeur, Fanny; Brocard, Lysiane; Chambaud, Clement; Grison, Magali S.; Paterlini, Andrea; Glavier, Marie; Hoornaert, Lucie; Joshi, Amit S.; Gontier, Etienne; Prinz, William A.; Jaillais, Yvon; Taly, Antoine; Campelo, Felix; Caillaud, Marie-Cecile; Bayer, Emmanuelle M.
• 署名单位: Centre National de la Recherche Scientifique (CNRS); CNRS - National Institute for Biology (INSB); Universite de Bordeaux; Universite de Bordeaux; Centre National de la Recherche Scientifique (CNRS); Institut National de la Sante et de la Recherche Medicale (Inserm); INRAE; University of Tennessee System; University of Tennessee Knoxville; University of Texas System; University of Texas Dallas; University of Texas Southwestern Medical Center; Centre National de la Recherche Scientifique (CNRS); Ecole Normale Superieure de Lyon (ENS de LYON); Universite Claude Bernard Lyon 1; INRAE; Centre National de la Recherche Scientifique (CNRS); CNRS - Institute of Chemistry (INC); Universite Paris Cite; Barcelona Institute of Science & Technology; Universitat Politecnica de Catalunya; Institut de Ciencies Fotoniques (ICFO); University of Edinburgh
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-8722
• DOI: 10.1126/science.adn4630
• 发表日期: 2024-11-01
• 页码: 538-545
• 关键词: endoplasmic-reticulum; cell plate; escrt-iii; membrane-proteins; arabidopsis; abscission; ultrastructure; constriction; bilayers; tension

摘要

Diverging from conventional cell division models, plant cells undergo incomplete division to generate plasmodesmata communication bridges between daughter cells. Although fundamental for plant multicellularity, the molecular events leading to bridge stabilization, as opposed to severing, remain unknown. Using electron tomography, we mapped the transition from cell plate fenestrae to plasmodesmata. We show that the endoplasmic reticulum (ER) connects daughter cells across fenestrae, and as the cell plate matures, fenestrae contract, causing the plasma membrane (PM) to mold around constricted ER tubes. The ER's presence prevents fenestrae fusion, forming plasmodesmata, whereas its absence results in closure. The ER-PM protein tethers MCTP3, MCTP4, and MCTP6 further stabilize nascent plasmodesmata during fenestrae contraction. Genetic deletion in Arabidopsis reduces plasmodesmata formation. Our findings reveal how plants undergo incomplete division to promote intercellular communication.