A wheat tandem kinase and NLR pair confers resistance to multiple fungal pathogens

• 成果类型: Article
• 署名作者: Lu, Ping; Zhang, Gaohua; Li, Jing; Gong, Zhen; Wang, Gaojie; Dong, Lingli; Zhang, Huaizhi; Guo, Guanghao; Su, Min; Wang, Ke; Wang, Yueming; Zhu, Keyu; Wu, Qiuhong; Chen, Yongxing; Li, Miaomiao; Huang, Baoge; Li, Beibei; Li, Wenling; Dong, Lei; Hou, Yikun; Cui, Xuejia; Fu, Hongkui; Qiu, Dan; Yuan, Chengguo; Li, Hongjie; Zhou, Jian-Min; Han, Guan-Zhu; Chen, Yuhang; Liu, Zhiyong
• 署名单位: Chinese Academy of Sciences; Institute of Genetics & Developmental Biology, CAS; Chinese Academy of Sciences; Institute of Genetics & Developmental Biology, CAS; Chinese Academy of Sciences; University of Chinese Academy of Sciences, CAS; Beijing University of Agriculture; Nanjing Normal University; Xianghu Laboratory; Yazhouwan National Laboratory; Hainan Seed Industry Laboratory
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-13190
• DOI: 10.1126/science.adp5469
• 发表日期: 2025-03-28
• 页码: 1418-1424
• 关键词: sequence alignment; EVOLUTION; resistosome; genomes; complex; blast; gene

摘要

Tandem kinase proteins underlie the innate immune systems of cereal plants, but how they initiate plant immune responses remains unclear. This report identifies wheat protein wheat tandem NBD 1 (WTN1), a noncanonical nucleotide-binding leucine-rich repeat (NLR) receptor featuring tandem nucleotide binding adaptor shared by APAF-1, plant R proteins, and CED-4 (NB-ARC) domains, required for WTK3-mediated disease resistance. Both WTK3 and its allelic variant Rwt4-known for conferring resistance to wheat powdery mildew and blast, respectively-are capable of recognizing the blast effector PWT4. They activate WTN1 to form calcium-permeable channels, akin to ZAR1 and Sr35. Thus, tandem kinase proteins and their associated NLRs operate as sensor-executor pairs against fungal pathogens. Additionally, evolutionary analyses reveal a coevolutionary trajectory of the tandem kinase-NLR module, highlighting their cooperative role in triggering plant immunity.