Bacterial pathogen deploys the iminosugar glycosyrin to manipulate plant glycobiology

• 成果类型: Article
• 署名作者: Sanguankiattichai, Nattapong; Chandrasekar, Balakumaran; Sheng, Yuewen; Hardenbrook, Nathan; Tabak, Werner W. A.; Drapal, Margit; Kaschani, Farnusch; Gruenwald-Gruber, Clemens; Krahn, Daniel; Buscaill, Pierre; Yamamoto, Suzuka; Kato, Atsushi; Nash, Robert; Fleet, George; Strasser, Richard; Fraser, Paul D.; Kaiser, Markus; Zhang, Peijun; Preston, Gail M.; van der Hoorn, Renier A. L.
• 署名单位: University of Oxford; Mahidol University; Diamond Light Source; University of Oxford; Wellcome Centre for Human Genetics; University of Duisburg Essen; University of London; Royal Holloway University London; University of Duisburg Essen; BOKU University; Dortmund University of Technology; Leibniz Association; Leibniz Institut fur Analytische Wissenschaften (ISAS); University of Toyama; University of Oxford; BOKU University
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-9125
• DOI: 10.1126/science.adp2433
• 发表日期: 2025-04-18
• 页码: 297-303
• 关键词: pseudomonas-syringae; escherichia-coli; beta-galactosidase; mass-spectrometry; gene-cluster; identification; database; platform; cloning; biosynthesis

摘要

The extracellular space (apoplast) in plants is a key battleground during microbial infections. To avoid recognition, the bacterial model phytopathogen Pseudomonas syringae pv. tomato DC3000 produces glycosyrin. Glycosyrin inhibits the plant-secreted beta-galactosidase BGAL1, which would otherwise initiate the release of immunogenic peptides from bacterial flagellin. Here, we report the structure, biosynthesis, and multifunctional roles of glycosyrin. High-resolution cryo-electron microscopy and chemical synthesis revealed that glycosyrin is an iminosugar with a five-membered pyrrolidine ring and a hydrated aldehyde that mimics monosaccharides. Glycosyrin biosynthesis was controlled by virulence regulators, and its production is common in bacteria and prevents flagellin recognition and alters the extracellular glycoproteome and metabolome of infected plants. These findings highlight a potentially wider role for glycobiology manipulation by plant pathogens across the plant kingdom.