RNA-mediated CRISPR-Cas13 inhibition through crRNA structural mimicry
成果类型：
Article
署名作者：
Hayes, Victoria M.; Zhang, Jun-Tao; Katz, Mark A.; Li, Yuelong; Kocsis, Benjamin; Brinkley, David M.; Jia, Ning; Meeske, Alexander J.
署名单位：
University of Washington; University of Washington Seattle; Southern University of Science & Technology; Southern University of Science & Technology
刊物名称：
SCIENCE
ISSN/ISSBN：
0036-8681
DOI：
10.1126/science.adr3656
发表日期：
2025-04-25
页码：
387-391
关键词：
摘要：
To circumvent CRISPR-Cas immunity, phages express anti-CRISPR factors that inhibit the expression or activities of Cas proteins. Whereas most anti-CRISPRs described to date are proteins, recently described small RNAs called RNA anti-CRISPRs (rAcrs) have sequence homology to CRISPR RNAs (crRNAs) and displace them from cognate Cas nucleases. In this work, we report the discovery of rAcrVIA1-a plasmid-encoded small RNA that inhibits the RNA-targeting CRISPR-Cas13 system in its natural host, Listeria seeligeri. We solved the cryo-electron microscopy structure of the Cas13-rAcr complex, which revealed that rAcrVIA1 adopts a fold nearly identical to crRNA despite sharing negligible sequence similarity. Collectively, our findings expand the diversity of rAcrs and reveal an example of immune antagonism through RNA structural mimicry.