Imaging-guided deep tissue in vivo sound printing

• 成果类型: Article
• 署名作者: Davoodi, Elham; Li, Jiahong; Ma, Xiaotian; Najafabadi, Alireza Hasani; Yoo, Jounghyun; Lu, Gengxi; Sani, Ehsan Shirzaei; Lee, Sunho; Montazerian, Hossein; Kim, Gwangmook; Williams, Jason; Yang, Jee Won; Zeng, Yushun; Li, Lei S.; Jin, Zhiyang; Sadri, Behnam; Nia, Shervin S.; Wang, Lihong V.; Hsiai, Tzung K.; Weiss, Paul S.; Zhou, Qifa; Khademhosseini, Ali; Wu, Di; Shapiro, Mikhail G.; Gao, Wei
• 署名单位: California Institute of Technology; California Institute of Technology; Rice University; Rice University; University of California System; University of California Los Angeles; University of California System; University of California Los Angeles
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-11137
• DOI: 10.1126/science.adt0293
• 发表日期: 2025-05-08
• 页码: 616-623
• 关键词: delivery; release

摘要

Three-dimensional printing offers promise for patient-specific implants and therapies but is often limited by the need for invasive surgical procedures. To address this, we developed an imaging-guided deep tissue in vivo sound printing (DISP) platform. By incorporating cross-linking agent-loaded low-temperature-sensitive liposomes into bioinks, DISP enables precise, rapid, on-demand cross-linking of diverse functional biomaterials using focused ultrasound. Gas vesicle-based ultrasound imaging provides real-time monitoring and allows for customized pattern creation in live animals. We validated DISP by successfully printing near diseased areas in the mouse bladder and deep within rabbit leg muscles in vivo, demonstrating its potential for localized drug delivery and tissue replacement. DISP's ability to print conductive, drug-loaded, cell-laden, and bioadhesive biomaterials demonstrates its versatility for diverse biomedical applications.