Self-organized tissue mechanics underlie embryonic regulation

• 成果类型: Article
• 署名作者: Caldarelli, Paolo; Chamolly, Alexander; Villedieu, Aurelien; Alegria-Prevot, Olinda; Phan, Carole; Gros, Jerome; Corson, Francis
• 署名单位: Pasteur Network; Universite Paris Cite; Institut Pasteur Paris; Centre National de la Recherche Scientifique (CNRS); CNRS - National Institute for Biology (INSB); Sorbonne Universite; Centre National de la Recherche Scientifique (CNRS); Universite PSL; Ecole Normale Superieure (ENS); Sorbonne Universite; Universite Paris Cite; Rockefeller University
• 刊物名称: Nature
• ISSN/ISSBN: 0028-6354
• DOI: 10.1038/s41586-024-07934-8
• 发表日期: 2024-10-24
• 关键词: primitive streak formation; cell intercalation; pattern-formation; MODEL; polarity; vg1; gastrulation; expression; SYSTEM; shape

摘要

Early amniote development is highly self-organized, capable of adapting to interference through local and long-range cell-cell interactions. This process, called embryonic regulation1, has been well illustrated in experiments on avian embryos, in which subdividing the epiblast disk into different parts not only redirects cell fates to eventually form a complete and well-proportioned embryo at its original location, but also leads to the self-organization of additional, fully formed embryos2,3 in the other separated parts. The cellular interactions underlying embryonic self-organization are widely believed to be mediated by molecular signals, yet the identity of such signals is unclear. Here, by analysing intact and mechanically perturbed quail embryos, we show that the mechanical forces that drive embryogenesis self-organize, with contractility locally self-activating and the ensuing tension acting as a long-range inhibitor. This mechanical feedback governs the persistent pattern of tissue flows that shape the embryo4-6 and also steers the concomitant emergence of embryonic territories by modulating gene expression, ensuring the formation of a single embryo under normal conditions, yet allowing the emergence of multiple, well-proportioned embryos after perturbations. Thus, mechanical forces act at the core of embryonic self-organization, shaping both tissues and gene expression to robustly yet plastically canalize early development. Mechanical forces act at the core of bird embryonic self-organization, shaping both tissues and gene expression to robustly yet plastically canalize early development.