Polyclonality overcomes fitness barriers in Apc-driven tumorigenesis

• 成果类型: Article
• 署名作者: Sadien, Iannish D.; Adler, Sam; Mehmed, Shenay; Bailey, Sasha; Sawle, Ashley; Couturier, Dominique-Laurent; Eldridge, Matthew; Adams, David J.; Kemp, Richard; Lourenco, Filipe C.; Winton, Douglas J.
• 署名单位: CRUK Cambridge Institute; Cancer Research UK; Francis Crick Institute; Wellcome Trust Sanger Institute
• 刊物名称: Nature
• ISSN/ISSBN: 0028-6335
• DOI: 10.1038/s41586-024-08053-0
• 发表日期: 2024-10-31
• 页码: 1196-1203
• 关键词: colorectal-cancer; stem-cells; clonal evolution; mouse model; origin; COMPETITION; expression; adenomas; leads

摘要

Loss-of-function mutations in the tumour suppressor APC are an initial step in intestinal tumorigenesis1,2. APC-mutant intestinal stem cells outcompete their wild-type neighbours through the secretion of Wnt antagonists, which accelerates the fixation and subsequent rapid clonal expansion of mutants3-5. Reports of polyclonal intestinal tumours in human patients and mouse models appear at odds with this process6,7. Here we combine multicolour lineage tracing with chemical mutagenesis in mice to show that a large proportion of intestinal tumours have a multiancestral origin. Polyclonal tumours retain a structure comprising subclones with distinct Apc mutations and transcriptional states, driven predominantly by differences in KRAS and MYC signalling. These pathway-level changes are accompanied by profound differences in cancer stem cell phenotypes. Of note, these findings are confirmed by introducing an oncogenic Kras mutation that results in predominantly monoclonal tumour formation. Further, polyclonal tumours have accelerated growth dynamics, suggesting a link between polyclonality and tumour progression. Together, these findings demonstrate the role of interclonal interactions in promoting tumorigenesis through non-cell autonomous pathways that are dependent on the differential activation of oncogenic pathways between clones. Multicolour lineage tracing and mutagenesis studies in a mouse model show that many intestinal tumours are polyclonal, with multiple clones exhibiting independent Apc mutations driven by differences in KRAS and MYC signalling.