A framework for neural organoids, assembloids and transplantation studies
成果类型：
Review
署名作者：
Pasca, Sergiu P.; Arlotta, Paola; Bateup, Helen S.; Camp, J. Gray; Cappello, Silvia; Gage, Fred H.; Knoblich, Juergen A.; Kriegstein, Arnold R.; Lancaster, Madeline A.; Ming, Guo-Li; Novarino, Gaia; Okano, Hideyuki; Parmar, Malin; Park, In-Hyun; Reiner, Orly; Song, Hongjun; Studer, Lorenz; Takahashi, Jun; Temple, Sally; Testa, Giuseppe; Treutlein, Barbara; Vaccarino, Flora M.; Vanderhaeghen, Pierre; Young-Pearse, Tracy
署名单位：
Stanford University; Stanford University; Harvard University; Harvard University; Massachusetts Institute of Technology (MIT); Broad Institute; University of California System; University of California Berkeley; University of California System; University of California Berkeley; Roche Holding; University of Basel; University of Munich; Max Planck Society; Salk Institute; Vienna Biocenter (VBC); Institute of Molecular Biotechnology (IMBA); Austrian Academy of Sciences; Medical University of Vienna; University of California System; University of California San Francisco; University of California System; University of California San Francisco; MRC Laboratory Molecular Biology; University of Cambridge; University of Pennsylvania; University of Pennsylvania; University of Pennsylvania; University of Pennsylvania; University of Pennsylvania; Institute of Science & Technology - Austria; Keio University; Lund University; Yale University; Yale University; Weizmann Institute of Science; Weizmann Institute of Science; University of Pennsylvania; Memorial Sloan Kettering Cancer Center; Kyoto University; University of Milan; Human Technopole; Yale University; Yale University; Flanders Institute for Biotechnology (VIB); Harvard University; Harvard Medical School; Harvard University Medical Affiliates; Brigham & Women's Hospital; Harvard University; Harvard Medical School; Harvard University
刊物名称：
Nature
ISSN/ISSBN：
0028-1470
DOI：
10.1038/s41586-024-08487-6
发表日期：
2025-03-13
页码：
315-320
关键词：
stem-cells
摘要：
As the field of neural organoids and assembloids expands, there is an emergent need for guidance and advice on designing, conducting and reporting experiments to increase the reproducibility and utility of these models. In this Perspective, we present a framework for the experimental process that encompasses ensuring the quality and integrity of human pluripotent stem cells, characterizing and manipulating neural cells in vitro, transplantation techniques and considerations for modelling human development, evolution and disease. As with all scientific endeavours, we advocate for rigorous experimental designs tailored to explicit scientific questions as well as transparent methodologies and data sharing to provide useful knowledge for current research practices and for developing regulatory standards.