Spatial mapping of transcriptomic plasticity in metastatic pancreatic cancer
成果类型：
Article
署名作者：
Pei, Guangsheng; Min, Jimin; Rajapakshe, Kimal I.; Branchi, Vittorio; Liu, Yunhe; Selvanesan, Benson Chellakkan; Thege, Fredrik; Sadeghian, Dorsay; Zhang, Daiwei; Cho, Kyung Serk; Chu, Yanshuo; Dai, Enyu; Han, Guangchun; Li, Mingyao; Yee, Cassian; Takahashi, Kazuki; Garg, Bharti; Tiriac, Herve; Bernard, Vincent; Semaan, Alexander; Grem, Jean L.; Caffrey, Thomas C.; Burks, Jared K.; Lowy, Andrew M.; Aguirre, Andrew J.; Grandgenett, Paul M.; Hollingsworth, Michael A.; Guerrero, Paola A.; Wang, Linghua; Maitra, Anirban
署名单位：
University of Texas System; UTMD Anderson Cancer Center; University of Texas System; UTMD Anderson Cancer Center; University of Texas System; UTMD Anderson Cancer Center; University of Pennsylvania; University of North Carolina; University of North Carolina Chapel Hill; University of North Carolina; University of North Carolina Chapel Hill; University of Texas System; UTMD Anderson Cancer Center; University of Texas System; UTMD Anderson Cancer Center; Harvard University; Harvard University Medical Affiliates; Dana-Farber Cancer Institute; Harvard University; Harvard Medical School; Harvard University; Massachusetts Institute of Technology (MIT); Broad Institute; University of California System; University of California San Diego; University of Texas System; UTMD Anderson Cancer Center; University of Bonn; University of Nebraska System; University of Nebraska Medical Center; University of Nebraska System; University of Nebraska Medical Center; University of Texas System; UTMD Anderson Cancer Center; University of Texas System; UTMD Anderson Cancer Center; Harvard University; Harvard University Medical Affiliates; Brigham & Women's Hospital; University of Texas System; UTMD Anderson Cancer Center; University of Texas System; UTMD Anderson Cancer Center; University of Texas System; UTMD Anderson Cancer Center
刊物名称：
Nature
ISSN/ISSBN：
0028-2998
DOI：
10.1038/s41586-025-08927-x
发表日期：
2025-06-05
关键词：
ductal adenocarcinoma
molecular subtypes
Heterogeneity
landscape
driver
microenvironment
differentiation
immunotherapy
fibroblasts
inhibition
摘要：
Patients with treatment-refractory pancreatic cancer often succumb to systemic metastases1, 2-3; however, the transcriptomic heterogeneity that underlies therapeutic recalcitrance remains understudied, particularly in a spatial context. Here we construct high-resolution maps of lineage states, clonal architecture and the tumour microenvironment (TME) using spatially resolved transcriptomics from 55 samples of primary tumour and metastases (liver, lung and peritoneum) collected from rapid autopsies of 13 people. We observe discernible transcriptomic shifts in cancer-cell lineage states as tumours transition from primary sites to organ-specific metastases, with the most pronounced intra-patient distinctions between liver and lung. Phylogenetic trees constructed from inferred copy number variations in primary and metastatic loci in each patient highlight diverse patient-specific evolutionary trajectories and clonal dissemination. We show that multiple tumour lineage states co-exist in each tissue, including concurrent metastatic foci in the same organ. Agnostic to tissue site, lineage states correlate with distinct TME features, such as the spatial proximity of TGFB1-expressing myofibroblastic cancer-associated fibroblasts (myCAFs) to aggressive 'basal-like' cancer cells, but not to cells in the 'classical' or 'intermediate' states. These findings were validated through orthogonal and cross-species analyses using mouse tissues and patient-derived organoids. Notably, basal-like cancer cells aligned with myCAFs correlate with plasma-cell exclusion from the tumour milieu, and neighbouring cell analyses suggest that CXCR4-CXCL12 signalling is the underlying basis for observed immune exclusion. Collectively, our findings underscore the profound transcriptomic heterogeneity and microenvironmental dynamics that characterize treatment-refractory pancreatic cancer.