Genomic and genetic insights into Mendel's pea genes

• 成果类型: Article
• 署名作者: Feng, Cong; Chen, Baizhi; Hofer, Julie; Shi, Yan; Jiang, Mei; Song, Bo; Cheng, Hong; Lu, Lu; Wang, Luyao; Howard, Alex; Bendahmane, Abdel; Fouchal, Anissa; Moreau, Carol; Sawada, Chie; LeSignor, Christine; Zhang, Cuijun; Vikeli, Eleni; Tsanakas, Georgios; Zhao, Hang; Cheema, Jitender; Barclay, J. Elaine; Hou, Junliang; Sayers, Liz; Wingen, Luzie; Vigouroux, Marielle; Vickers, Martin; Ambrose, Mike; Dalmais, Marion; Higuera-Poveda, Paola; Li, Pengfeng; Yuan, Quan; Spanner, Rebecca; Horler, Richard; Wouters, Roland; Chundakkad, Smitha; Wu, Tian; Zhao, Xiaoxiao; Li, Xiuli; Sun, Yuchen; Huang, Zejian; Wu, Zhen; Deng, Xing Wang; Steuernagel, Burkhard; Domoney, Claire; Ellis, Noel; Chayut, Noam; Cheng, Shifeng
• 署名单位: Guangdong Laboratory for Lingnan Modern Agriculture; Chinese Academy of Agricultural Sciences; Agriculture Genomes Institute at Shenzhen, CAAS; UK Research & Innovation (UKRI); Biotechnology and Biological Sciences Research Council (BBSRC); John Innes Center; INRAE; Universite Paris Saclay; Universite Paris Cite; INRAE; Universite Bourgogne Europe; INRAE; Institut Agro; AgroSup Dijon; European Molecular Biology Laboratory (EMBL); European Bioinformatics Institute; University of Minnesota System; University of Minnesota Twin Cities; Peking University; Peking University
• 刊物名称: Nature
• ISSN/ISSBN: 0028-2997
• DOI: 10.1038/s41586-025-08891-6
• 发表日期: 2025-06-26
• 关键词: green cotyledon gene; cle peptides; plant; pxy; association; population; EVOLUTION; encodes; alleles; complex

摘要

Mendel1 studied in detail seven pairs of contrasting traits in pea (Pisum sativum), establishing the foundational principles of genetic inheritance. Here we investigate the genetic architecture that underlies these traits and uncover previously undescribed alleles for the four characterized Mendelian genes2, 3, 4, 5, 6-7, including a rare revertant of Mendel's white-flowered a allele. Primarily, we focus on the three remaining uncharacterized traits and find that (1) an approximately 100-kb genomic deletion upstream of the Chlorophyll synthase (ChlG) gene disrupts chlorophyll biosynthesis through the generation of intergenic transcriptional fusion products, conferring the yellow pod phenotype of gp mutants; (2) a MYB gene with an upstream Ogre element insertion and a CLE peptide-encoding gene with an in-frame premature stop codon explain the v and p alleles, which disrupt secondary cell wall thickening and lignification, resulting in the parchmentless, edible-pod phenotype; and (3) a 5-bp exonic deletion in a CIK-like co-receptor kinase gene, in combination with a genetic modifier locus, is associated with the fasciated stem (fa) phenotype. Furthermore, we characterize genes and alleles associated with diverse agronomic traits, such as axil ring anthocyanin pigmentation, seed size and the 'semi-leafless' form. This study establishes a foundation for fundamental research, education in biology and genetics, and pea breeding practices.