2025

最新文章

• De novo-designed pMHC binders facilitate T cell-mediated cytotoxicity toward cancer cells AI翻译
  作者：Johansen, Kristoffer Haurum; Wolff, Darian Stephan; Scapolo, Beatrice; Fernandez-Quintero, Monica L.; Christensen, Charlotte Risager; Loeffler, Johannes R.; Rivera-de-Torre, Esperanza; Overath, Max D.; Munk, Kamilla Kjaergaard; Morell, Oliver; Viuff, Marie Christine; Lacunza, Inigo; Englund, Alberte T. Damm; Due, Mathilde; Gharpure, Anant; Forli, Stefano; Pardo, Carlos Rodriguez; Tamhane, Tripti; Andersen, Emma Qingjie; Bjoernsson, Kasper Haldrup; Fernandes, Jordan Sylvester; Voss, Lasse Frank; Thumtecho, Suthimon; Ward, Andrew B.; Ormhoj, Maria; Hadrup, Sine Reker; Jenkins, Timothy P.
  作者单位：Technical University of Denmark; Technical University of Denmark; Scripps Research Institute
  摘要：The recognition of intracellular antigens by CD8+ T cells through T cell receptors (TCRs) is central for adaptive immunity against infections and cancer. However, the identification of TCRs from patient material remains complex. We present a rapid de novo minibinder (miBd) design platform leveraging state-of-the-art generative models to engineer miBds targeting the cancer-associated peptide-bound major histocompatibility complex (pMHC) SLLMWITQC/HLA-A*02:01 (NY-ESO-1). Incorporating in silico ...
• Global patterns and drivers of untracked industrial fishing in coastal marine protected areas AI翻译
  作者：Seguin, Raphael; Le Manach, Frederic; Devillers, Rodolphe; Velez, Laure; Mouillot, David
  作者单位：Institut de Recherche pour le Developpement (IRD); Centre National de la Recherche Scientifique (CNRS); Ifremer; Universite de Montpellier; Universite de Montpellier; University of La Reunion
  摘要：Marine protected areas (MPAs) are expanding worldwide, but industrial fishing within their boundaries remains poorly understood. By combining vessel Global Positioning System data with satellite imagery, we detected industrial fishing in 47% of coastal MPAs worldwide between 2022 and 2024, with two-thirds of vessel detections (67%) untracked by public monitoring. These untracked vessels were most prevalent (80%) in MPAs with the most restrictive management category, which aims to prohibit all ...
• Flat optics produces quantum graphs AI翻译
  作者：Shcherbakov, Maxim R.
  作者单位：University of California System; University of California Irvine
• Midline assembloids reveal regulators of human axon guidance AI翻译
  作者：Onesto, Massimo M.; Amin, Neal D.; Pan, Chenjie; Chen, Xiaoyu; Kim, Ji-il; Reis, Noah; Kanton, Sabina; Valencia, Alfredo M.; Hudacova, Zuzana; McQueen, James P.; Tessier-Lavigne, Marc; Pasca, Sergiu P.
  作者单位：Stanford University; Stanford Medicine; Stanford University; Stanford University; Stanford University
  摘要：Organizers orchestrate cell patterning and axon guidance in the developing nervous system. Although nonhuman models have led to fundamental discoveries about floor plate (FP)-mediated midline organization, an experimental model of the human FP would enable insights into human neurodevelopment and midline connectivity. Here, we developed organoids resembling human FP (hFpOs) and assembled them with human spinal cord organoids (hSpOs) to generate midline assembloids (hMAs). We demonstrate that h...
• A new age of molecular chirality Advanced optical approaches reshape the frontiers of chiral sensing AI翻译
  作者：Smirnova, Olga
  作者单位：Leibniz Association; Max Born Institute for Nonlinear Optics & Short Term Spectroscopy; Technical University of Berlin; Technion Israel Institute of Technology
• ASB7 is a negative regulator of H3K9me3 homeostasis AI翻译
  作者：Zhou, Liwen; Chen, Zhenxuan; Zou, Yezi; Zhang, Xia; Wang, Zifeng; Zhu, Hongwen; Lin, Jiahui; Huang, Ziyao; Zheng, Lisi; Chen, Jiali; Xie, Miner; Zhang, Meifang; Zhang, Ruhua; Zhu, Minglu; Wang, Ziwen; Zhou, Hu; Gao, Song; Yin, Yuxin; Wu, Yuanzhong; Kang, Tiebang
  作者单位：Sun Yat Sen University; State Key Lab Oncology South China; Sun Yat Sen University; Chinese Academy of Sciences; Shanghai Institute of Materia Medica, CAS; South China University of Technology; Peking University
  摘要：The maintenance of histone H3 lysine 9 trimethylation (H3K9me3) involves the recognition of preexisting modifications by heterochromatin protein 1 (HP1), which recruits the methyltransferase suppressor of variegation 3-9 homolog 1 (SUV39H1) to methylate the adjacent newly incorporated histones, establishing a positive feedback loop. However, how this positive feedback is restricted to maintain H3K9me3 homeostasis remains largely unknown. We performed an unbiased genome-scale CRISPR-Cas9 screen...
• Atomic explosion AI翻译
  作者：Cho, Adrian
• Tissue-integrated bionic knee restores versatile legged movement after amputation AI翻译
  作者：Shu, Tony; Levine, Daniel; Yeon, Seong Ho; Chun, Ethan; Shallal, Christopher C.; McCullough, John; Branemark, Rickard; Carty, Matthew J.; Ferrone, Marco; Boerhout, Sean; Ko, Alexander; Sullivan, Corey L.; Zhu, Gloria; Nawrot, Michael; Carney, Matthew; Wieschhoff, Ged; Friedman, Gabriel; Herr, Hugh
  作者单位：Massachusetts Institute of Technology (MIT); Massachusetts Institute of Technology (MIT); Massachusetts Institute of Technology (MIT); Massachusetts Institute of Technology (MIT); Massachusetts Institute of Technology (MIT); University of Gothenburg; Harvard University; Harvard University Medical Affiliates; Brigham & Women's Hospital; Harvard University; Harvard University Medical Affiliates; Brigham & Women's Hospital; Harvard University; Harvard University Medical Affiliates; Brigham & Women's Hospital; Harvard University; Harvard University Medical Affiliates; Massachusetts General Hospital
  摘要：Lower-extremity prostheses have evolved through mechanical redesigns that prioritize improved cyclic locomotion. However, this limited approach to limb restoration has precluded necessary progress toward recovering the versatile acyclic movements that constitute the remainder of human athleticism. We present an osseointegrated mechanoneural prosthesis that incorporates modified hard and soft tissues along with permanently implanted hardware in a neuroembodied design. We developed a biomimetic ...
• Discrete spatiotemporal encoding of striatal dopamine transmission AI翻译
  作者：Yee, Andrew G.; Liao, Yini; Muntean, Brian S.; Ford, Christopher P.
  作者单位：University of Colorado System; University of Colorado Anschutz; University System of Georgia; Augusta University
  摘要：Dopamine (DA) transmission critically regulates diverse, basal ganglia-dependent behaviors by activating distinct subtypes of G protein-coupled receptors. Spatially broad DA release has been extensively characterized, but how DA transmission affects striatal function at a subcellular scale remains poorly understood. Using 2P imaging and whole-cell electrophysiology, we defined the spatiotemporal properties of DA transmission onto striatal indirect pathway spiny projection neurons. Sparse activ...
• The intracellular Ca2+ sensitivity of transmitter release in glutamatergic neocortical boutons AI翻译
  作者：Bornschein, Grit; Brachtendorf, Simone; Reinert, Anja; Eshra, Abdelmoneim; Kraft, Robert; Hirrlinger, Johannes; Eilers, Jens; Hallermann, Stefan; Schmidt, Hartmut
  作者单位：Leipzig University
  摘要：Synaptotagmin-1 (Syt1) and Syt2 are the main calcium (Ca2+) sensors triggering synchronous release in the brain. In this work, we studied the mechanisms mediating Syt1-triggered release from neocortical synapses. We measured the Ca2+ dependency of release in layer 5 pyramidal neuron synapses by laser photolysis of caged Ca2+. Release had high Ca2+ affinity and positive cooperativity. Measurements at cerebellar Purkinje cell synapses and kinetic models indicate substantial differences compared ...