作者：Christou, Nina-Eleni; Apostolopoulou, Virginia; Melo, Diogo V. M.; Ruppert, Matthias; Fadini, Alisia; Henkel, Alessandra; Sprenger, Janina; Oberthuer, Dominik; Guenther, Sebastian; Pateras, Anastasios; Mashhour, Aida Rahmani; Yefanov, Oleksandr M.; Galchenkova, Marina; Reinke, Patrick Y. A.; Kremling, Viviane; Scheer, T. Emilie S.; Lange, Esther R.; Middendorf, Philipp; Schubert, Robin; De Zitter, Elke; Lumbao-Conradson, Koya; Herrmann, Jonathan; Rahighi, Simin; Kunavar, Ajda; Beale, Emma V.; Beale, John H.; Cirelli, Claudio; Johnson, Philip J. M.; Dworkowski, Florian; Ozerov, Dmitry; Bertrand, Quentin; Wranik, Maximilian; Bacellar, Camila; Bajt, Sasa; Wakatsuki, Soichi; Sellberg, Jonas A.; Huse, Nils; Turk, Dusan; Chapman, Henry N.; Lane, Thomas J.
作者单位：Helmholtz Association; Deutsches Elektronen-Synchrotron (DESY); European XFEL; University of Hamburg; Imperial College London; Communaute Universite Grenoble Alpes; Universite Grenoble Alpes (UGA); CEA; Centre National de la Recherche Scientifique (CNRS); Stanford University; United States Department of Energy (DOE); SLAC National Accelerator Laboratory; Stanford University; University of Ljubljana; Swiss Federal Institutes of Technology Domain; Paul Scherrer Institute; Stanford University; United States Department of Energy (DOE); SLAC National Accelerator Laboratory; Royal Institute of Technology; Slovenian Academy of Sciences & Arts (SASA); Jozef Stefan Institute; University of Hamburg; Horiba Ltd.; University of Cambridge; University of Colorado System; University of Colorado Anschutz Medical Campus; Chapman University System; Chapman University; National Institute of Chemistry - Slovenia
摘要：Photolyase is an enzyme that uses light to catalyze DNA repair. To capture the reaction intermediates involved in the enzyme's catalytic cycle, we conducted a time-resolved crystallography experiment. We found that photolyase traps the excited state of the active cofactor, flavin adenine dinucleotide (FAD), in a highly bent geometry. This excited state performs electron transfer to damaged DNA, inducing repair. We show that the repair reaction, which involves the lysis of two covalent bonds, o...
作者：Maestre-Reyna, Manuel; Wang, Po-Hsun; Nango, Eriko; Hosokawa, Yuhei; Saft, Martin; Furrer, Antonia; Yang, Cheng-Han; Putu, Eka Putra Gusti Ngurah; Wu, Wen-Jin; Emmerich, Hans-Joachim; Caramello, Nicolas; Franz-Badur, Sophie; Yang, Chao; Engilberge, Sylvain; Wranik, Maximilian; Glover, Hannah Louise; Weinert, Tobias; Wu, Hsiang-Yi; Lee, Cheng-Chung; Huang, Wei-Cheng; Huang, Kai-Fa; Chang, Yao-Kai; Liao, Jiahn-Haur; Weng, Jui-Hung; Gad, Wael; Chang, Chiung-Wen; Pang, Allan H.; Yang, Kai-Chun; Lin, Wei-Ting; Chang, Yu-Chen; Gashi, Dardan; Beale, Emma; Ozerov, Dmitry; Nass, Karol; Knopp, Gregor; Johnson, Philip J. M.; Cirelli, Claudio; Milne, Chris; Bacellar, Camila; Sugahara, Michihiro; Owada, Shigeki; Joti, Yasumasa; Yamashita, Ayumi; Tanaka, Rie; Tanaka, Tomoyuki; Luo, Fangjia; Tono, Kensuke; Zarzycka, Wiktoria; Mueller, Pavel; Alahmad, Maisa Alkheder; Bezold, Filipp; Fuchs, Valerie; Gnau, Petra; Kiontke, Stephan; Korf, Lukas; Reithofer, Viktoria; Rosner, Christian Joshua; Seiler, Elisa Marie; Watad, Mohamed; Werel, Laura; Spadaccini, Roberta; Yamamoto, Junpei; Iwata, So; Zhong, Dongping; Standfuss, Joerg; Royant, Antoine; Bessho, Yoshitaka; Essen, Lars-Oliver; Tsai, Ming-Daw
作者单位：Academia Sinica - Taiwan; National Taiwan University; RIKEN; Tohoku University; University of Osaka; Philipps University Marburg; Swiss Federal Institutes of Technology Domain; Paul Scherrer Institute; European Synchrotron Radiation Facility (ESRF); University of Hamburg; University System of Ohio; Ohio State University; Centre National de la Recherche Scientifique (CNRS); Communaute Universite Grenoble Alpes; Universite Grenoble Alpes (UGA); CEA; Japan Synchrotron Radiation Research Institute; Kyoto University; Universite Paris Saclay; Centre National de la Recherche Scientifique (CNRS); CEA; University of Sannio; University System of Ohio; Ohio State University; Shanghai Jiao Tong University; National Taiwan University; University of California System; University of California San Diego
摘要：Photolyases, a ubiquitous class of flavoproteins, use blue light to repair DNA photolesions. In this work, we determined the structural mechanism of the photolyase-catalyzed repair of a cyclobutane pyrimidine dimer (CPD) lesion using time-resolved serial femtosecond crystallography (TR-SFX). We obtained 18 snapshots that show time-dependent changes in four reaction loci. We used these results to create a movie that depicts the repair of CPD lesions in the picosecond-to-nanosecond range, follow...
