Unsaturated fat alters clock phosphorylation to align rhythms to the season in mice

• 成果类型: Article
• 署名作者: Levine, Daniel C.; Reeh, Rasmus H.; McMahon, Thomas; Mandrup-Poulsen, Thomas; Fu, Ying-Hui; Ptacek, Louis J.
• 署名单位: University of California System; University of California San Francisco; University of Copenhagen; University of California System; University of California San Francisco; University of California System; University of California San Francisco; University of California System; University of California San Francisco
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-8075; 1095-9203
• DOI: 10.1126/science.adp3065
• 发表日期: 2025-10-23
• 页码: eadp3065
• 关键词: gene-expression; circadian clock; adipose-tissue; temperature; Mutation

摘要

The circadian clock maintains synchrony between biological processes and light/dark cycles by integrating environmental cues. How the clock adapts to seasonal variations in the environment is incompletely understood. We found that a high-fat diet increased phosphorylation of the clock protein PERIOD2 (PER2) on serine 662 (S662), which was necessary and sufficient for regulating phase shifting of daily locomotor activity to entrain to seasonal light cycles. PER2-S662 phosphorylation correlated with genome-wide expression pathways that regulate polyunsaturated fatty acid (PUFA) conversion into oxylipins in the hypothalamus. Partial hydrogenation of dietary PUFAs increased hypothalamic PER2-S662 phosphorylation and entrainment to a summer photoperiod in control mice, but not in mice for which PER2-S662 could not be phosphorylated. PER2-S662 phosphorylation is influenced by, and alters the regulation of, unsaturated fat to control circadian phase shifting across the seasons.