Localized glutamine leakage drives the spatial structure of root microbial colonization

• 成果类型: Article
• 署名作者: Tsai, Huei-Hsuan; Tang, Yuanjie; Jiang, Lingmin; Xu, Xiaoyan; Tendon, Valerie Denervaud; Pang, Jia; Jia, Yanyan; Wippel, Kathrin; Vacheron, Jordan; Keel, Christoph; Andersen, Tonni Grube; Geldner, Niko; Zhou, Feng
• 署名单位: University of Lausanne; Chinese Academy of Sciences; Center for Excellence in Molecular Plant Sciences, CAS; Chinese Academy of Sciences; University of Chinese Academy of Sciences, CAS; Chinese Academy of Sciences; Center for Excellence in Molecular Plant Sciences, CAS; Max Planck Society; University of Lausanne
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-8075; 1095-9203
• DOI: 10.1126/science.adu4235
• 发表日期: 2025-10-02
• 页码: eadu4235
• 关键词: GRAM-NEGATIVE BACTERIA; ACIDIC AMINO-ACIDS; dependent regulation; gene-expression; arabidopsis; chemotaxis; TRANSFORMATION; PSEUDOMONADS; suppression; homeostasis

摘要

Plant roots release exudates to encourage microbiome assembly, which influences the function and stress resilience of plants. How specific exudates drive spatial colonization patterns remains largely unknown. In this study, we demonstrate that endodermal Casparian strips-forming the root's extracellular diffusion barrier-restrict nutrient leakage into the rhizosphere, coinciding with and controlling spatial colonization patterns of rhizobacteria. We find that vasculature-derived glutamine leakage is a major bacterial chemoattractant and enhancer of proliferation, defining a previously unknown pathway for root exudate formation. Bacteria defective in amino acid chemoperception display reduced attraction toward leakage sites, and roots with Casparian strip defects display bacterial overproliferation, dependent on bacterial capacity for amino acid metabolization. Associated chronic immune stimulation suggests that endodermal nutrient restriction is crucial for regulating microbial colonization and assembly, limiting excessive proliferation that could compromise plant health.