Xanthomonas coordinates type III-type II effector synergy by activating fruit-ripening pathway

• 成果类型: Article
• 署名作者: Phan, Trang Thi-Thu; Streit, Rodrigo Silva Araujo; Minsavage, Gerald V.; Kilian, Joachim; Aguilera, Paloma de los Angeles; Wang, Nan; Brich, Nicolas; Morbitzer, Robert; von Roepenack-Lahaye, Edda; Charleux, Brice; Szurek, Boris; de Giuseppe, Priscila Oliveira; Licciardello, Concetta; Jones, Jeffrey B.; Teixeira, Paulo J. P. L.; Persinoti, Gabriela Felix; Murakami, Mario Tyago; Liu, Chang; Grau, Jan; Lahaye, Thomas
• 署名单位: Eberhard Karls University of Tubingen; State University System of Florida; University of Florida; Eberhard Karls University of Tubingen; INRAE; CIRAD; Institut Agro; Institut de Recherche pour le Developpement (IRD); Universite de Montpellier; Consiglio per la Ricerca in Agricoltura e L'analisi Dell'economia Agraria (CREA); Universidade de Sao Paulo; Martin Luther University Halle Wittenberg; Eberhard Karls University of Tubingen; University Hohenheim
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-8075; 1095-9203
• DOI: 10.1126/science.adz9239
• 发表日期: 2025-12-18
• 页码: 1292-1298
• 关键词: gene-expression; PV. CITRI; plant; genome; bacteria; sequence; features; package; systems; LIFE

摘要

Plant cell walls harbor vast carbohydrate reserves, yet how pathogens unlock them remains unclear. We show that the citrus canker pathogen Xanthomonas citri subsp. citri (Xcc) mobilizes cell wall sugars by hijacking a fruit-ripening program through the type III effector PthA4, which activates the ripening coordinator CsLOB1. CsLOB1 induces approximately 100 genes, many encoding enzymes involved in cell wall breakdown. In the nonfruiting species Nicotiana benthamiana, CsLOB1 likewise promotes Xanthomonas growth, showing that its activity is not strictly dependent on a ripening program. Transcriptomics and reporter assays revealed PthA4-dependent activation of the Xcc xylan CUT system, triggered by host-derived xylose and including a type II-secreted xylanase. Thus, PthA4-driven cell wall remodeling activates bacterial xylan use, establishing a TIII-TII effector feedforward loop that fuels Xcc proliferation.