pSTAT3 activation of Foxl2 initiates the female pathway underlying temperature- dependent sex determination

• 成果类型: Article
• 署名作者: Wu, Pengfei; Wang, Xifeng; Ge, Chutian; Jin, Lin; Ding, Zihan; Liu, Fang; Zhang, Ju; Gao, Fei; Du, Weiguo
• 署名单位: Chinese Academy of Sciences; Institute of Zoology, CAS; Chinese Academy of Sciences; University of Chinese Academy of Sciences, CAS; Zhejiang Wanli University; Zhejiang Wanli University; Chinese Academy of Sciences; Institute of Zoology, CAS
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-14640
• DOI: 10.1073/pnas.2401752121
• 发表日期: 2024-09-10
• 关键词: gene-transcription; molecular-cloning; stat3 inhibitor; expression; promoter; ovaries; binding; cancer; smads

摘要

Ovarian development was traditionally recognized as a default sexual outcome and therefore received much less scientific attention than testis development. In turtles with temperature- dependent sex determination (TSD), how the female pathway is initiated to induce ovary development remains unknown. In this study, we have found that phosphorylation of the signal transducer and activator of transcription 3 (pSTAT3) and Foxl2 exhibit temperature- dependent sexually dimorphic patterns and tempo- spatial Inhibition of pSTAT3 at a female- producing temperature of 31 degrees C induces 64.7% female- to- male sex reversal, whereas activation of pSTAT3 at a male- producing temperature of 26 degrees C triggers 75.6% male- to- female sex reversal. In addition, pSTAT3 induced by inhibition or activation of pSTAT3. This study has established a direct genetic link between warm temperature- induced STAT3 phosphorylation and female pathway initiation in a TSD system, highlighting the critical role of pSTAT3 in the cross talk