High- level succinic acid production by overexpressing a magnesium transporter in Mannheimia succiniciproducens

• 成果类型: Article
• 署名作者: Kim, Ji Yeon; Lee, Jong An; Ahn, Jung Ho; Lee, Sang Yup
• 署名单位: Korea Advanced Institute of Science & Technology (KAIST); Korea Advanced Institute of Science & Technology (KAIST); Korea Advanced Institute of Science & Technology (KAIST); Korea Advanced Institute of Science & Technology (KAIST); Korea Institute of Science & Technology (KIST)
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-13667
• DOI: 10.1073/pnas.2407455121
• 发表日期: 2024-09-10
• 关键词: escherichia-coli; anaerobiospirillum-succiniciproducens; neutralizing agent; gene knockout; fermentation; carboxykinase; bacterium; SYSTEM; ratio; ions

摘要

Succinic acid (SA), a dicarboxylic acid of industrial importance, can be efficiently produced by metabolically engineered Mannheimia succiniciproducens. Although the importance of magnesium (Mg2+) ion on SA production has been evident from our previous studies, the role of Mg2+ ion remains largely unexplored. In this study, we investigated the impact of Mg2+ ion on SA production and developed a hyper- SA producing strain of M. succiniciproducens by reconstructing the Mg2+ ion transport system. To achieve this, optimal alkaline neutralizer comprising Mg2+ ion was developed and the physiological effect of Mg2+ ion was analyzed. Subsequently, the Mg2+ ion transport system was reconstructed by introducing an efficient Mg2+ ion transporter from Salmonella enterica. A high- inoculum fed- batch fermentation of the final engineered strain produced 152.23 +/- 0.99 g/L of SA, with a maximum productivity of 39.64 +/- 0.69 g/L/h. These findings highlight the importance of Mg2+ ions and transportation system optimization in succinic acid production by M. succiniciproducens.