Diversity and evolution of nitric oxide reduction in bacteria and archaea

• 成果类型: Article
• 署名作者: Murali, Ranjani; Pace, Laura A.; Sanford, Robert A.; Ward, L. M.; Lynes, Mackenzie M.; Hatzenpichler, Roland; Lingappa, Usha F.; Fischer, Woodward W.; Gennis, Robert B.; Hemp, James
• 署名单位: University of Illinois System; University of Illinois Urbana-Champaign; California Institute of Technology; Nevada System of Higher Education (NSHE); University of Nevada Las Vegas; University of Illinois System; University of Illinois Urbana-Champaign; Smith College; California Institute of Technology; Montana State University System; Montana State University Bozeman; Montana State University System; Montana State University Bozeman; Montana State University System; Montana State University Bozeman; University of California System; University of California Berkeley
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-13039
• DOI: 10.1073/pnas.2316422121
• 发表日期: 2024-06-25
• 关键词: cytochrome a(1)-like hemoprotein; active-site residue; c-oxidase; rhodothermus-marinus; aerobic respiration; cbb(3) oxidase; nitrous-oxide; input channel; proton pump; heme-a

摘要

Nitrous oxide is a potent greenhouse gas whose production is catalyzed by nitric oxide reductase (NOR) members of the heme - copper oxidoreductase (HCO) enzyme superfamily. We identified several previously uncharacterized HCO families, four of which (eNOR, sNOR, gNOR, and nNOR) appear to perform NO reduction. These families have novel active - site structures and several have conserved proton channels, suggesting that they might be able to couple NO reduction to energy conservation. We isolated and biochemically characterized a member of the eNOR family from the bacterium Rhodothermus marinus and found that it performs NO reduction. These recently identified NORs exhibited broad phylogenetic and environmental distributions, greatly expanding the diversity of microbes in nature capable of NO reduction. Phylogenetic analyses further demonstrated that NORs evolved multiple times independently from oxygen reductases, supporting the view that complete denitrification evolved after aerobic respiration.