A CUC1/auxin genetic module links cell polarity to patterned tissue growth and leaf shape diversity in crucifer plants

• 成果类型: Article
• 署名作者: Hu, Zi-Liang; Wilson-Sanchez, David; Bhatia, Neha; Rast-Somssich, Madlen I.; Wu, Anhui; Vlad, Daniela; McGuire, Liam; Nikolov, Lachezar A.; Laufs, Patrick; Gan, Xiangchao; Laurent, Stefan; Runions, Adam; Tsiantis, Miltos
• 署名单位: Max Planck Society; Universite Paris Saclay; INRAE; AgroParisTech; University of Calgary; Heinrich Heine University Dusseldorf; University of Oxford; University of London; King's College London; Indiana University System; Indiana University Bloomington; Nanjing Agricultural University; BioNTech SE
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-11610
• DOI: 10.1073/pnas.2321877121
• 发表日期: 2024-06-25
• 关键词: arabidopsis-thaliana; pin phosphorylation; auxin; MODEL; expression; transport; EVOLUTION; FRAMEWORK; motifs; SYSTEM

摘要

How tissue - level information encoded by fields of regulatory gene activity is translated into the patterns of cell polarity and growth that generate the diverse shapes of different species remains poorly understood. Here, we investigate this problem in the case of leaf shape differences between Arabidopsis thaliana , which has simple leaves, and its relative Cardamine hirsuta that has complex leaves divided into leaflets. We show that patterned expression of the transcription factor CUP - SHAPED COTYLEDON1 in C. hirsuta (ChCUC1) is a key determinant of leaf shape differences between the two species. Through inducible genetic perturbations, time - lapse imaging of growth, and computational modeling, we find that ChCUC1 provides instructive input into auxin - based leaf margin patterning. This input arises via transcriptional regulation of multiple auxin homeostasis components, including direct activation of WAG kinases that are known to regulate the polarity of PIN - FORMED auxin transporters. Thus, we have uncovered a mechanism that bridges biological scales by linking spatially distributed and species - specific transcription factor expression to cell - level polarity and growth, to shape diverse leaf forms.