Loss of a gluconeogenic muscle enzyme contributed to adaptive metabolic traits in hummingbirds

• 成果类型: Article
• 署名作者: Osipova, Ekaterina; Barsacchi, Rico; Brown, Tom; Sadanandan, Keren; Gaede, Andrea H.; Monte, Amanda; Jarrells, Julia; Moebius, Claudia; Pippel, Martin; Altshuler, Douglas L.; Winkler, Sylke; Bickle, Marc; Baldwin, Maude W.; Hiller, Michael
• 署名单位: Max Planck Society; Max Planck Society; Leibniz Association; Senckenberg Gesellschaft fur Naturforschung (SGN); Goethe University Frankfurt; Technische Universitat Dresden; Max Planck Society; University of British Columbia; University of London; University of London Royal Veterinary College; Max Planck Society
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-11726
• DOI: 10.1126/science.abn7050
• 发表日期: 2023-01-13
• 页码: 185-190
• 关键词: hovering flight; EVOLUTION; sugar; bird

摘要

Hummingbirds possess distinct metabolic adaptations to fuel their energy-demanding hovering flight, but the underlying genomic changes are largely unknown. Here, we generated a chromosome-level genome assembly of the long-tailed hermit and screened for genes that have been specifically inactivated in the ancestral hummingbird lineage. We discovered that FBP2 (fructose-bisphosphatase 2), which encodes a gluconeogenic muscle enzyme, was lost during a time period when hovering flight evolved. We show that FBP2 knockdown in an avian muscle cell line up-regulates glycolysis and enhances mitochondrial respiration, coincident with an increased mitochondria number. Furthermore, genes involved in mitochondrial respiration and organization have up-regulated expression in hummingbird flight muscle. Together, these results suggest that FBP2 loss was likely a key step in the evolution of metabolic muscle adaptations required for true hovering flight.