CRISPR technology: A decade of genome editing is only the beginning

• 成果类型: Review
• 署名作者: Wang, Joy Y.; Doudna, Jennifer A.
• 署名单位: University of California System; University of California Berkeley; University of California System; University of California Berkeley; University of California System; University of California Berkeley; Howard Hughes Medical Institute; University of California System; University of California Berkeley; University of California System; University of California Berkeley; United States Department of Energy (DOE); Lawrence Berkeley National Laboratory; University of California System; University of California San Francisco; The J David Gladstone Institutes
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-11098
• DOI: 10.1126/science.add8643
• 发表日期: 2023-01-20
• 页码: 251-+
• 关键词: peptide-mediated delivery; one-step generation; off-target; guide-rna; cas9 ribonucleoprotein; base editors; human-cells; dna; gene; mice

摘要

The advent of clustered regularly interspaced short palindromic repeat (CRISPR) genome editing, coupled with advances in computing and imaging capabilities, has initiated a new era in which genetic diseases and individual disease susceptibilities are both predictable and actionable. Likewise, genes responsible for plant traits can be identified and altered quickly, transforming the pace of agricultural research and plant breeding. In this Review, we discuss the current state of CRISPR-mediated genetic manipulation in human cells, animals, and plants along with relevant successes and challenges and present a roadmap for the future of this technology.