Brassinosteroid gene regulatory networks at cellular resolution in the Arabidopsis root

• 成果类型: Article
• 署名作者: Nolan, Trevor M.; Vukasinovic, Nemanja; Hsu, Che-Wei; Zhang, Jingyuan; Vanhoutte, Isabelle; Shahan, Rachel; Taylor, Isaiah W.; Greenstreet, Laura; Heitz, Matthieu; Afanassiev, Anton; Wang, Ping; Szekely, Pablo; Brosnan, Aiden; Yin, Yanhai; Schiebinger, Geoffrey; Ohler, Uwe; Russinova, Eugenia; Benfey, Philip N.
• 署名单位: Duke University; Ghent University; Flanders Institute for Biotechnology (VIB); Humboldt University of Berlin; Helmholtz Association; Max Delbruck Center for Molecular Medicine; Howard Hughes Medical Institute; Duke University; University of British Columbia; Iowa State University; Humboldt University of Berlin
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-12330
• DOI: 10.1126/science.adf4721
• 发表日期: 2023-03-31
• 页码: 1314-+
• 关键词: plant-growth; transcription factors; extracellular domain; class-i; expression; meristem; kinase; biosynthesis; differentiation; perception

摘要

Brassinosteroids are plant steroid hormones that regulate diverse processes, such as cell division and cell elongation, through gene regulatory networks that vary in space and time. By using time series single-cell RNA sequencing to profile brassinosteroid-responsive gene expression specific to different cell types and developmental stages of the Arabidopsis root, we identified the elongating cortex as a site where brassinosteroids trigger a shift from proliferation to elongation associated with increased expression of cell wall-related genes. Our analysis revealed HOMEOBOX FROM ARABIDOPSIS THALIANA 7 (HAT7) and GT-2-LIKE 1 (GTL1) as brassinosteroid-responsive transcription factors that regulate cortex cell elongation. These results establish the cortex as a site of brassinosteroid-mediated growth and unveil a brassinosteroid signaling network regulating the transition from proliferation to elongation, which illuminates aspects of spatiotemporal hormone responses.