Rhythmic cilia changes support SCN neuron coherence in circadian clock

• 成果类型: Article
• 署名作者: Tu, Hai-Qing; Li, Sen; Xu, Yu-Ling; Zhang, Yu-Cheng; Li, Pei-Yao; Liang, Li-Yun; Song, Guang-Ping; Jian, Xiao-Xiao; Wu, Min; Song, Zeng-Qing; Li, Ting -Ting; Hu, Huai -Bin; Yuan, Jin-Feng; Shen, Xiao-Lin; Li, Jia-Ning; Han, Qiu-Ying; Wang, Kai; Zhang, Tao; Zhou, Tao; Li, Ai -Ling; Zhang, Xue-Min; Li, Hui-Yan
• 署名单位: Fudan University; Academy of Military Medical Sciences - China
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-12106
• DOI: 10.1126/science.abm1962
• 发表日期: 2023-06-02
• 页码: 972-979
• 关键词: transcriptional architecture; suprachiasmatic nucleus; synchrony; component; disease

摘要

The suprachiasmatic nucleus (SCN) drives circadian clock coherence through intercellular coupling, which is resistant to environmental perturbations. We report that primary cilia are required for intercellular coupling among SCN neurons to maintain the robustness of the internal clock in mice. Cilia in neuromedin S-producing (NMS) neurons exhibit pronounced circadian rhythmicity in abundance and length. Genetic ablation of ciliogenesis in NMS neurons enabled a rapid phase shift of the internal clock under jet-lag conditions. The circadian rhythms of individual neurons in cilia-deficient SCN slices lost their coherence after external perturbations. Rhythmic cilia changes drive oscillations of Sonic Hedgehog (Shh) signaling and clock gene expression. Inactivation of Shh signaling in NMS neurons phenocopied the effects of cilia ablation. Thus, cilia-Shh signaling in the SCN aids intercellular coupling.