Spatially resolved single-cell translatomics at molecular resolution

• 成果类型: Article
• 署名作者: Zeng, Hu; Huang, Jiahao; Ren, Jingyi; Wang, Connie Kangni; Tang, Zefang; Zhou, Haowen; Zhou, Yiming; Shi, Hailing; Aditham, Abhishek; Sui, Xin; Chen, Hongyu; Lo, Jennifer A.; Wang, Xiao
• 署名单位: Massachusetts Institute of Technology (MIT); Harvard University; Massachusetts Institute of Technology (MIT); Broad Institute; Harvard University; Massachusetts Institute of Technology (MIT); Broad Institute; Massachusetts Institute of Technology (MIT)
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-13307
• DOI: 10.1126/science.add3067
• 发表日期: 2023-06-30
• 页码: 1337-+
• 关键词: rna translation; DYNAMICS; protein; quantification

摘要

The precise control of messenger RNA (mRNA) translation is a crucial step in posttranscriptional gene regulation of cellular physiology. However, it remains a challenge to systematically study mRNA translation at the transcriptomic scale with spatial and single-cell resolution. Here, we report the development of ribosome-bound mRNA mapping (RIBOmap), a highly multiplexed three-dimensional in situ profiling method to detect cellular translatome. RIBOmap profiling of 981 genes in HeLa cells revealed cell cycle-dependent translational control and colocalized translation of functional gene modules. We mapped 5413 genes in mouse brain tissues, yielding spatially resolved single-cell translatomic profiles for 119,173 cells and revealing cell type-specific and brain region-specific translational regulation, including translation remodeling during oligodendrocyte maturation. Our method detected widespread patterns of localized translation in neuronal and glial cells in intact brain tissue networks.