Multiplex CRISPR editing of wood for sustainable fiber production

• 成果类型: Article
• 署名作者: Sulis, Daniel B.; Jiang, Xiao; Yang, Chenmin; Marques, Barbara M.; Matthews, Megan L.; Miller, Zachary; Lan, Kai; Cofre-Vega, Carlos; Liu, Baoguang; Sun, Runkun; Sederoff, Henry; Bing, Ryan G.; Sun, Xiaoyan; Williams, Cranos M.; Jameel, Hasan; Phillips, Richard; Chang, Hou-min; Peszlen, Ilona; Huang, Yung-Yun; Li, Wei; Kelly, Robert M.; Sederoff, Ronald R.; Chiang, Vincent L.; Barrangou, Rodolphe; Wang, Jack P.
• 署名单位: North Carolina State University; North Carolina State University; North Carolina State University; North Carolina State University; University of Illinois System; University of Illinois Urbana-Champaign; Beihua University; North Carolina State University; North Carolina State University; North Carolina State University; Northeast Forestry University - China; North Carolina State University
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-12303
• DOI: 10.1126/science.add4514
• 发表日期: 2023-07-14
• 页码: 216-+
• 关键词: lignin biosynthesis; enzyme

摘要

The domestication of forest trees for a more sustainable fiber bioeconomy has long been hindered by the complexity and plasticity of lignin, a biopolymer in wood that is recalcitrant to chemical and enzymatic degradation. Here, we show that multiplex CRISPR editing enables precise woody feedstock design for combinatorial improvement of lignin composition and wood properties. By assessing every possible combination of 69,123 multigenic editing strategies for 21 lignin biosynthesis genes, we deduced seven different genome editing strategies targeting the concurrent alteration of up to six genes and produced 174 edited poplar variants. CRISPR editing increased the wood carbohydrate-to-lignin ratio up to 228% that of wild type, leading to more-efficient fiber pulping. The edited wood alleviates a major fiber-production bottleneck regardless of changes in tree growth rate and could bring unprecedented operational efficiencies, bioeconomic opportunities, and environmental benefits.