Control of histone demethylation by nuclear-localized α-ketoglutarate dehydrogenase

• 成果类型: Article
• 署名作者: Huang, Fei; Luo, Xiao; Ou, Yang; Gao, Zhaoxu; Tang, Qiming; Chu, Zhenzhen; Zhu, Xinguang; He, Yuehui
• 署名单位: Peking University; Chinese Academy of Sciences; Center for Excellence in Molecular Plant Sciences, CAS; Peking University; Chinese Academy of Sciences; Center for Excellence in Molecular Plant Sciences, CAS
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-11283
• DOI: 10.1126/science.adf8822
• 发表日期: 2023-07-14
• 页码: 179-+
• 关键词: flowering-locus-c; gene-expression; transcription factors; floral transition; domain protein; arabidopsis; complex; dna; methylation; sequence

摘要

Methylations on nucleosomal histones play fundamental roles in regulating eukaryotic transcription. Jumonji C domain-containing histone demethylases (JMJs) dynamically control the level of histone methylations. However, how JMJ activity is generally regulated is unknown. We found that the tricarboxylic acid cycle-associated enzyme alpha-ketoglutarate (alpha-KG) dehydrogenase (KGDH) entered the nucleus, where it interacted with various JMJs to regulate alpha-KG-dependent histone demethylations by JMJs, and thus controlled genome-wide gene expression in plants. We show that nuclear targeting is regulated by environmental signals and that KGDH is enriched at thousands of loci in Arabidopsis thaliana. Chromatin-bound KGDH catalyzes a-KG decarboxylation and thus may limit its local availability to KGDH-coupled JMJs, inhibiting histone demethylation. Thus, our results uncover a regulatory mechanism for histone demethylations by JMJs.