Climbing fiber multi-innervation of mouse Purkinje dendrites with arborization common to human

• 成果类型: Article
• 署名作者: Busch, Silas E.; Hansel, Christian
• 署名单位: University of Chicago; University of Chicago
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-8827
• DOI: 10.1126/science.adi1024
• 发表日期: 2023-07-28
• 页码: 420-+
• 关键词: complex-spike; plasticity; cells; input; potentiation; elimination; DEPRESSION; signals; voltage; synapse

摘要

Canonically, each Purkinje cell (PC) in the adult cerebellum receives only one climbing fiber (CF) from the inferior olive. Underlying current theories of cerebellar function is the notion that this highly conserved one-to-one relationship renders Purkinje dendrites into a single computational compartment. However, we discovered that multiple primary dendrites are a near-universal morphological feature in humans. Using tract tracing, immunolabeling, and in vitro electrophysiology, we found that in mice similar to 25% of mature multibranched cells receive more than one CF input. Two-photon calcium imaging in vivo revealed that separate dendrites can exhibit distinct response properties to sensory stimulation, indicating that some multibranched cells integrate functionally independent CF-receptive fields. These findings indicate that PCs are morphologically and functionally more diverse than previously thought.