Yolk sac cell atlas reveals multiorgan functions during human early development

• 成果类型: Article
• 署名作者: Goh, Issac; Botting, Rachel A.; Rose, Antony; Webb, Simone; Engelbert, Justin; Gitton, Yorick; Stephenson, Emily; Quiroga Londono, Mariana; Mather, Michael; Mende, Nicole; Imaz-Rosshandler, Ivan; Yang, Lu; Horsfall, Dave; Basurto-Lozada, Daniela; Chipampe, Nana-Jane; Rook, Victoria; Lee, Jimmy Tsz Hang; Ton, Mai-Linh; Keitley, Daniel; Mazin, Pavel; Vijayabaskar, M. S.; Hannah, Rebecca; Gambardella, Laure; Green, Kile; Ballereau, Stephane; Inoue, Megumi; Tuck, Elizabeth; Lorenzi, Valentina; Kwakwa, Kwasi; Alsinet, Clara; Olabi, Bayanne; Miah, Mohi; Admane, Chloe; Popescu, Dorin-Mirel; Acres, Meghan; Dixon, David; Ness, Thomas; Coulthard, Rowen; Lisgo, Steven; Henderson, Deborah J.; Dann, Emma; Suo, Chenqu; Kinston, Sarah J.; Park, Jong-eun; Polanski, Krzysztof; Marioni, John; van Dongen, Stijn; Meyer, Kerstin B.; de Bruijn, Marella; Palis, James; Behjati, Sam; Laurenti, Elisa; Wilson, Nicola K.; Vento-Tormo, Roser; Chedotal, Alain; Bayraktar, Omer; Roberts, Irene; Jardine, Laura; Goettgens, Berthold; Teichmann, Sarah A.; Haniffa, Muzlifah
• 署名单位: Wellcome Trust Sanger Institute; Newcastle University - UK; Sorbonne Universite; Institut National de la Sante et de la Recherche Medicale (Inserm); Centre National de la Recherche Scientifique (CNRS); MRC Laboratory Molecular Biology; University of Cambridge; Newcastle University - UK; Newcastle Upon Tyne Hospitals NHS Foundation Trust; Newcastle University - UK; Korea Advanced Institute of Science & Technology (KAIST); European Molecular Biology Laboratory (EMBL); European Bioinformatics Institute; CRUK Cambridge Institute; University of Cambridge; University of Oxford; University of Rochester; University of Cambridge; University of Oxford; University of Cambridge; Newcastle University - UK; Newcastle Upon Tyne Hospitals NHS Foundation Trust; Newcastle University - UK; Newcastle Upon Tyne Hospitals NHS Foundation Trust
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-8601
• DOI: 10.1126/science.add7564
• 发表日期: 2023-08-18
• 页码: 747-+
• 关键词: hematopoietic stem-cells; progenitors; emergence; endothelium; macrophage; microglia

摘要

The extraembryonic yolk sac (YS) ensures delivery of nutritional support and oxygen to the developing embryo but remains ill-defined in humans. We therefore assembled a comprehensive multiomic reference of the human YS from 3 to 8 postconception weeks by integrating single-cell protein and gene expression data. Beyond its recognized role as a site of hematopoiesis, we highlight roles in metabolism, coagulation, vascular development, and hematopoietic regulation. We reconstructed the emergence and decline of YS hematopoietic stem and progenitor cells from hemogenic endothelium and revealed a YS-specific accelerated route to macrophage production that seeds developing organs. The multiorgan functions of the YS are superseded as intraembryonic organs develop, effecting a multifaceted relay of vital functions as pregnancy proceeds.