Alcanivorax borkumensis biofilms enhance oil degradation by interfacial tubulation

• 成果类型: Article
• 署名作者: Prasad, M.; Obana, N.; Lin, S. -Z.; Zhao, S.; Sakai, K.; Blanch-Mercader, C.; Prost, J.; Nomura, N.; Rupprecht, J. -F.; Fattaccioli, J.; Utada, A. S.
• 署名单位: University of Tsukuba; University of Tsukuba; University of Tsukuba; Centre National de la Recherche Scientifique (CNRS); CNRS - Institute of Physics (INP); Aix-Marseille Universite; Sorbonne Universite; Universite PSL; Ecole Normale Superieure (ENS); Centre National de la Recherche Scientifique (CNRS); Universite PSL; UNICANCER; Institut Curie; Centre National de la Recherche Scientifique (CNRS); Sorbonne Universite; CNRS - Institute of Chemistry (INC); National University of Singapore; University of Tsukuba; University of Sheffield
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-8420
• DOI: 10.1126/science.adf3345
• 发表日期: 2023-08-18
• 页码: 748-753
• 关键词: marine-bacteria; biodegradation; environment; ecology; GROWTH

摘要

During the consumption of alkanes, Alcanivorax borkumensis will form a biofilm around an oil droplet, but the role this plays during degradation remains unclear. We identified a shift in biofilm morphology that depends on adaptation to oil consumption: Longer exposure leads to the appearance of dendritic biofilms optimized for oil consumption effected through tubulation of the interface. In situ microfluidic tracking enabled us to correlate tubulation to localized defects in the interfacial cell ordering. We demonstrate control over droplet deformation by using confinement to position defects, inducing dimpling in the droplets. We developed a model that elucidates biofilm morphology, linking tubulation to decreased interfacial tension and increased cell hydrophobicity.