Structural insights into histone binding and nucleosome assembly by chromatin assembly factor-1

• 成果类型: Article
• 署名作者: Liu, Chao-Pei; Yu, Zhenyu; Xiong, Jun; Hu, Jie; Song, Aoqun; Ding, Dongbo; Yu, Cong; Yang, Na; Wang, Mingzhu; Yu, Juan; Hou, Peini; Zeng, Kangning; Li, Zhenyu; Zhang, Zhuqiang; Zhang, Xinzheng; Li, Wei; Zhang, Zhiguo; Zhu, Bing; Li, Guohong; Xu, Rui-Ming
• 署名单位: Chinese Academy of Sciences; Chinese Academy of Sciences; Institute of Biophysics, CAS; Chinese Academy of Sciences; University of Chinese Academy of Sciences, CAS; Nankai University; Nankai University; Anhui University; Chinese Academy of Sciences; University of Chinese Academy of Sciences, CAS; Chinese Academy of Sciences; University of Chinese Academy of Sciences, CAS; Columbia University; NewYork-Presbyterian Hospital; NewYork-Presbyterian Hospital; Columbia University; Chinese Academy of Sciences; Institute of Biophysics, CAS
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-11678
• DOI: 10.1126/science.add8673
• 发表日期: 2023-08-25
• 页码: 850-+
• 关键词: dna-replication; chaperone caf-1; subunit; complex; protein; inheritance; h3-h4; pcna; p60; Visualization

摘要

Chromatin inheritance entails de novo nucleosome assembly after DNA replication by chromatin assembly factor-1 (CAF-1). Yet direct knowledge about CAF-1's histone binding mode and nucleosome assembly process is lacking. In this work, we report the crystal structure of human CAF-1 in the absence of histones and the cryo-electron microscopy structure of CAF-1 in complex with histones H3 and H4. One histone H3-H4 heterodimer is bound by one CAF-1 complex mainly through the p60 subunit and the acidic domain of the p150 subunit. We also observed a dimeric CAF-1-H3-H4 supercomplex in which two H3-H4 heterodimers are poised for tetramer assembly and discovered that CAF-1 facilitates right-handed DNA wrapping of H3-H4 tetramers. These findings signify the involvement of DNA in H3-H4 tetramer formation and suggest a right-handed nucleosome precursor in chromatin replication.