Molecular programs of regional specification and neural stem cell fate progression in macaque telencephalon

• 成果类型: Article
• 署名作者: Micali, Nicola; Ma, Shaojie; Li, Mingfeng; Kim, Suel-Kee; Mato-Blanco, Xoel; Sindhu, Suvimal Kumar; Arellano, Jon I.; Gao, Tianliuyun; Shibata, Mikihito; Gobeske, Kevin T.; Duque, Alvaro; Santpere, Gabriel; Sestan, Nenad; Rakic, Pasko
• 署名单位: Yale University; Huazhong University of Science & Technology; Pompeu Fabra University; Barcelona Biomedical Research Park; Hospital del Mar Research Institute; Hospital del Mar; Yale University; Yale University; Yale University
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-11042
• DOI: 10.1126/science.adf3786
• 发表日期: 2023-10-13
• 页码: 171-+
• 关键词: genome-wide association; human brain-development; insights; differentiation; transcriptome; arealization; expression; neocortex; neurons; cortex

摘要

During early telencephalic development, intricate processes of regional patterning and neural stem cell (NSC) fate specification take place. However, our understanding of these processes in primates, including both conserved and species-specific features, remains limited. Here, we profiled 761,529 single-cell transcriptomes from multiple regions of the prenatal macaque telencephalon. We deciphered the molecular programs of the early organizing centers and their cross-talk with NSCs, revealing primate-biased galanin-like peptide (GALP) signaling in the anteroventral telencephalon. Regional transcriptomic variations were observed along the frontotemporal axis during early stages of neocortical NSC progression and in neurons and astrocytes. Additionally, we found that genes associated with neuropsychiatric disorders and brain cancer risk might play critical roles in the early telencephalic organizers and during NSC progression.